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p53 antibody [DO1]

Anti-p53 antibody [DO1] used in Western Blot (WB). GTX70214
Anti-p53 antibody [DO1] used in IHC (Paraffin sections) (IHC-P). GTX70214
Anti-p53 antibody [DO1] used in Immunoprecipitation (IP). GTX70214
Anti-p53 antibody [DO1] used in Western Blot (WB). GTX70214
Anti-p53 antibody [DO1] used in Western Blot (WB). GTX70214
Anti-p53 antibody [DO1] used in Western Blot (WB). GTX70214
Anti-p53 antibody [DO1] used in Western Blot (WB). GTX70214
Anti-p53 antibody [DO1] used in IHC (Paraffin sections) (IHC-P). GTX70214
Anti-p53 antibody [DO1] used in IHC (Paraffin sections) (IHC-P). GTX70214

Cat No. GTX70214

Host

Mouse

Clonality

Monoclonal

Clone Name

DO1

Isotype

IgG2a

Application

WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ELISA

Reactivity

Human, Mouse
Package
200 μl ($329)
PRODUCT

Summary

p53 antibody recognizes p53 protein, which has a predicted molecular weight of 44 kDa but is typically detected by western blot at 53 kDa. p53, known widely as a tumor suppressor, plays critical roles in various cellular processes including cell cycle regulation, DNA repair, and apoptosis. p53 is mutated in many human cancers.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF Assay dependent
IHC-P 1:1000
IHC-Fr Assay dependent
FACS Assay dependent
IP 1:100-1:500
ELISA Assay dependent

Note :

IHC-P
Antigen retrieval can be done with EDTA at pH8.0 - Some customers have stated that the product works well without antigen retrieval.

Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

293T , A431 , HCT116 , 293T (0.5 μM doxorubicin treatment for 24 hr) , HCT116(30 μM cisplatin treatment for 24 hr)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.25 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human p53 expressed in E. coli.

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_370118

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tumor protein p53 , BCC7 , BMFS5 , LFS1 , P53 , TRP53

Cellular Localization

Cytoplasm , Nucleus , Endoplasmic reticulum , Mitochondrion matrix

Background

This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). [provided by RefSeq, Dec 2016]

Database

Research Area

DATA IMAGES

Anti-p53 antibody [DO1] used in Western Blot (WB). GTX70214

GTX70214 WB Image

Non-transfected (–) and transfected (+) HCT116 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [DO1] (GTX70214) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-p53 antibody [DO1] used in IHC (Paraffin sections) (IHC-P). GTX70214

GTX70214 IHC-P Image

p53 antibody [DO1] detects p53 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human pancreatic cancer.
p53 stained by p53 antibody [DO1] (GTX70214) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-p53 antibody [DO1] used in Immunoprecipitation (IP). GTX70214

GTX70214 IP Image

Immunoprecipitation of p53 protein from 293T whole cell extracts using 5 μg of p53 antibody [DO1] (GTX70214).
Western blot analysis was performed using p53 antibody [D01] (GTX70214).
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.

Anti-p53 antibody [DO1] used in Western Blot (WB). GTX70214

GTX70214 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [DO1] (GTX70214) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-p53 antibody [DO1] used in Western Blot (WB). GTX70214

GTX70214 WB Image

Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [DO1] (GTX70214) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-p53 antibody [DO1] used in Western Blot (WB). GTX70214

GTX70214 WB Image

293T whole cell extracts (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [DO1] (GTX70214) diluted at 1:40000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-p53 antibody [DO1] used in Western Blot (WB). GTX70214

GTX70214 WB Image

Untreated (–) and treated (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [DO1] (GTX70214) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-p53 antibody [DO1] used in IHC (Paraffin sections) (IHC-P). GTX70214

GTX70214 IHC-P Image

p53 antibody [DO1] detects p53 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
p53 stained by p53 antibody [DO1] (GTX70214) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-p53 antibody [DO1] used in IHC (Paraffin sections) (IHC-P). GTX70214

GTX70214 IHC-P Image

p53 antibody [DO1] detects p53 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
p53 stained by p53 antibody [DO1] (GTX70214) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

p53 antibody [DO1] Cat No. GTX70214

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2014.PMID: 24933654
Date : Anonymous submitted on 06-May-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : OS-RC-2 cells
Amount used : 30μg
Blocking : 25°C, 1Hr
Primary Antibody : 1:5000, 4°C, 16Hr
Application Reference
Clone Reference
SDS
PBS.pdf