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p53 antibody [DO1]

KO/KD Orthogonal
Anti-p53 antibody [DO1] used in Western Blot (WB). GTX70214
Anti-p53 antibody [DO1] used in IHC (Paraffin sections) (IHC-P). GTX70214
Anti-p53 antibody [DO1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70214
Anti-p53 antibody [DO1] used in IHC (Paraffin sections) (IHC-P). GTX70214
Anti-p53 antibody [DO1] used in Western Blot (WB). GTX70214
Anti-p53 antibody [DO1] used in Immunoprecipitation (IP). GTX70214
Anti-p53 antibody [DO1] used in Western Blot (WB). GTX70214
Anti-p53 antibody [DO1] used in IHC (Paraffin sections) (IHC-P). GTX70214
Anti-p53 antibody [DO1] used in Western Blot (WB). GTX70214
Anti-p53 antibody [DO1] used in IHC (Paraffin sections) (IHC-P). GTX70214
Anti-p53 antibody [DO1] used in IHC (Paraffin sections) (IHC-P). GTX70214
Anti-p53 antibody [DO1] used in IHC (Paraffin sections) (IHC-P). GTX70214
Anti-p53 antibody [DO1] used in Western Blot (WB). GTX70214
Anti-p53 antibody [DO1] used in Western Blot (WB). GTX70214

Cat. No. GTX70214

Reference 37
2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 DO1

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ELISA

Reactivity

 Human, Mouse
Package
200 μl ($399)
See all p53 products
PRODUCT

Summary

p53 antibody recognizes p53 protein, which has a predicted molecular weight of 44 kDa but is typically detected by western blot at 53 kDa. p53, known widely as a tumor suppressor, plays critical roles in various cellular processes including cell cycle regulation, DNA repair, and apoptosis. p53 is mutated in many human cancers.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF Assay dependent
IHC-P 1:1000
IHC-Fr Assay dependent
FACS Assay dependent
IP 1:100-1:500
ELISA Assay dependent

Note :

IHC-P
Antigen retrieval can be done with EDTA at pH8.0 - Some customers have stated that the product works well without antigen retrieval.
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

293T , A431 , HCT116 , 293T (0.5 μM doxorubicin treatment for 24 hr) , HCT116(30 μM cisplatin treatment for 24 hr)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.25 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human p53 expressed in E. coli.

Purification

Protein A purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_370118

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tumor protein p53 , BCC7 , BMFS5 , LFS1 , P53 , TRP53

Cellular Localization

Cytoplasm , Nucleus , Endoplasmic reticulum , Mitochondrion matrix

Background

This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). [provided by RefSeq, Dec 2016]

Database

Research Area

DATA IMAGES

Anti-p53 antibody [DO1] used in Western Blot (WB). GTX70214

GTX70214 WB Image

KO/KD

Non-transfected (–) and transfected (+) HCT116 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [DO1] (GTX70214) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-p53 antibody [DO1] used in IHC (Paraffin sections) (IHC-P). GTX70214

GTX70214 IHC-P Image

p53 antibody [DO1] detects p53 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human pancreatic cancer.
p53 stained by p53 antibody [DO1] (GTX70214) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-p53 antibody [DO1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70214

GTX70214 ICC/IF Image

Orthogonal

p53 antibody [DO1] detects p53 protein at nucleus by immunofluorescent analysis.
Sample: Mock and treated HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: p53 stained by p53 antibody [DO1] (GTX70214) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-p53 antibody [DO1] used in IHC (Paraffin sections) (IHC-P). GTX70214

GTX70214 IHC-P Image

p53 antibody [DO1] detects p53 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
p53 stained by p53 antibody [DO1] (GTX70214) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-p53 antibody [DO1] used in Western Blot (WB). GTX70214

GTX70214 WB Image

293T whole cell extracts (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [DO1] (GTX70214) diluted at 1:40000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-p53 antibody [DO1] used in Immunoprecipitation (IP). GTX70214

GTX70214 IP Image

Immunoprecipitation of p53 protein from 293T whole cell extracts using 5 μg of p53 antibody [DO1] (GTX70214).
Western blot analysis was performed using p53 antibody [D01] (GTX70214).
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.

Anti-p53 antibody [DO1] used in Western Blot (WB). GTX70214

GTX70214 WB Image

Orthogonal

Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [DO1] (GTX70214) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-p53 antibody [DO1] used in IHC (Paraffin sections) (IHC-P). GTX70214

GTX70214 IHC-P Image

p53 antibody [DO1] detects p53 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human NCI-H1299 xenograft.
p53 stained by p53 antibody [DO1] (GTX70214) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-p53 antibody [DO1] used in Western Blot (WB). GTX70214

GTX70214 WB Image

Orthogonal

Untreated (–) and treated (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [DO1] (GTX70214) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-p53 antibody [DO1] used in IHC (Paraffin sections) (IHC-P). GTX70214

GTX70214 IHC-P Image

p53 antibody [DO1] detects p53 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
p53 stained by p53 antibody [DO1] (GTX70214) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-p53 antibody [DO1] used in IHC (Paraffin sections) (IHC-P). GTX70214

GTX70214 IHC-P Image

p53 antibody [DO1] detects p53 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human SW480 xenograft.
p53 stained by p53 antibody [DO1] (GTX70214) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-p53 antibody [DO1] used in IHC (Paraffin sections) (IHC-P). GTX70214

GTX70214 IHC-P Image

p53 antibody [DO1] detects p53 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human oral carcinoma.
p53 stained by p53 antibody [DO1] (GTX70214) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-p53 antibody [DO1] used in Western Blot (WB). GTX70214

GTX70214 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [DO1] (GTX70214) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-p53 antibody [DO1] used in Western Blot (WB). GTX70214

GTX70214 WB Image

The data was published in the journal PLoS One in 2014. PMID: 24933654

REFERENCE

Application Reference

Phan TTT et al. Cell Death Discov 2023; 9 (1) : 340 Tumor suppressor p53 mediates interleukin-6 expression to enable cancer cell evasion of genotoxic stress.
Lin RC et al. Oncol Rep 2023; 50 (3) Polo?like kinase 1 selective inhibitor BI2536 (dihydropteridinone) disrupts centrosome homeostasis via ATM?ERK cascade in adrenocortical carcinoma.
Xu P et al. Cell Prolif 2023; 56 (2) : e13358 AMPK regulates homeostasis of invasion and viability in trophoblasts by redirecting glucose metabolism: Implications for pre-eclampsia.
Leu JD et al. Int J Mol Sci 2022; 23 (22) BPR0C261, An Analogous of Microtubule Disrupting Agent D-24851 Enhances the Radiosensitivity of Human Non-Small Cell Lung Cancer Cells via p53-Dependent and p53-Independent Pathways.
Chiang-Wen Lee et al. Molecules 2022; 27 (2) Naringenin Induces ROS-Mediated ER Stress, Autophagy, and Apoptosis in Human Osteosarcoma Cell Lines.
Application : WB
Reactivity : Human
Show More +
Submit a Reference

REVIEW

p53 antibody [DO1] Cat. No. GTX70214

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 2 users reviews)
Anti-p53 antibody [DO1] used in Western Blot (WB). GTX70214
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : U2OS cells
Amount used : 30 μg
Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 12Hr
Lane Description : untreated-5 μM cisplatin treatment for 24hr.
Anti-p53 antibody [DO1] used in Western Blot (WB). GTX70214
Date : Anonymous submitted on 06-May-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : OS-RC-2 cells
Amount used : 30μg
Blocking : 25°C, 1Hr
Primary Antibody : 1:5000, 4°C, 16Hr
Submit a Review
Application Reference
Phan TTT et al. Cell Death Discov 2023; 9 (1):340 Tumor suppressor p53 mediates interleukin-6 expression to enable cancer cell evasion of genotoxic stress.
Lin RC et al. Oncol Rep 2023; 50 (3) Polo?like kinase 1 selective inhibitor BI2536 (dihydropteridinone) disrupts centrosome homeostasis via ATM?ERK cascade in adrenocortical carcinoma.
Xu P et al. Cell Prolif 2023; 56 (2):e13358 AMPK regulates homeostasis of invasion and viability in trophoblasts by redirecting glucose metabolism: Implications for pre-eclampsia.
Leu JD et al. Int J Mol Sci 2022; 23 (22) BPR0C261, An Analogous of Microtubule Disrupting Agent D-24851 Enhances the Radiosensitivity of Human Non-Small Cell Lung Cancer Cells via p53-Dependent and p53-Independent Pathways.
Chiang-Wen Lee et al. Molecules 2022; 27 (2) Naringenin Induces ROS-Mediated ER Stress, Autophagy, and Apoptosis in Human Osteosarcoma Cell Lines.
Application : WB
Reactivity : Human
Wang LY et al. Int J Mol Sci 2022; 23 (5) The Anti-Proliferative and Apoptotic Effects of Rutaecarpine on Human Esophageal Squamous Cell Carcinoma Cell Line CE81T/VGH In Vitro and In Vivo.
Application : WB
Reactivity : Human
Yang C et al. Oncol Lett 2022; 23 (4):139 NRBP1 negatively regulates SALL4 to reduce the invasion and migration, promote apoptosis and increase the sensitivity to chemotherapy drugs of breast cancer cells.
Application : WB
Reactivity : Human
Lee CW et al. Molecules 2022; 27 (2):373 Naringenin Induces ROS-Mediated ER Stress, Autophagy, and Apoptosis in Human Osteosarcoma Cell Lines.
Application : WB
Reactivity : Human
Li R et al. Invest Ophthalmol Vis Sci 2021; 62 (9):2 Molecular Hydrogen Attenuated N-methyl-N-Nitrosourea Induced Corneal Endothelial Injury by Upregulating Anti-Apoptotic Pathway.
Application : ICC/IF
Reactivity : Human
Wang Y et al. Aging (Albany NY) 2020; 12 (20):20184-20197 Interleukin-6 knockout reverses macrophage differentiation imbalance and alleviates cardiac dysfunction in aging mice.
Zeng M et al. Cancer Manag Res 2020; 12:8801-8811 The Anti-Apoptotic Role of EBV-LMP1 in Lymphoma Cells.
Application : WB
Reactivity : Human
Huang YY et al. BMC Vet Res 2020; 16 (1):193 Inhibiting autophagy potentiates the antitumor efficacy of Euphorbia royleana for canine mammary gland tumors.
Application : WB
Huang HY et al. J Cell Mol Med 2020; Progesterone receptor membrane component 1 is involved in oral cancer cell metastasis.
Application : WB
Reactivity : Human
Hsu WH et al. Aging (Albany NY) 2020; 12 (11):10556-10577 T1-11, an adenosine derivative, ameliorates aging-related behavioral physiology and senescence markers in aging mice.
Application : WB
Reactivity : Human
Ye D et al. Toxicology 2020; 435:152422 Inhibition of cardiomyocyte differentiation of human induced pluripotent stem cells by Ribavirin: Implication for its cardiac developmental toxicity.
Application : WB
Reactivity : Human
Chen CY et al. Front Oncol 2020; 10:216 Hydroxygenkwanin Inhibits Class I HDAC Expression and Synergistically Enhances the Antitumor Activity of Sorafenib in Liver Cancer Cells.
Application : WB
Reactivity : Human
Wang YY et al. Biochem Pharmacol 2020; 171:113713 Orabase-formulated gentian violet effectively improved oral potentially malignant disorder in vitro and in vivo.
Application : WB
Reactivity : Human
Jingwen Li et al. RSC Adv 2019; 9 (67):39082-39089 New spirobisnaphthalenes from an endolichenic fungus strain CGMCC 3.15192 and their anticancer effects through the P53-P21 pathway.
Application : WB
Reactivity : Human
Zhang Y et al. Oncol Lett 2019; 17 (1):127-134 Apoptosis of mouse myeloma cells induced by curcumin via the Notch3-p53 signaling axis.
Celegato M et al. Cancer Lett 2019; (Epub) A novel small-molecule inhibitor of the human papillomavirus E6-p53 interaction that reactivates p53 function and blocks cancer cells growth.
Application : WB
Reactivity : Human
Chueh-Hsuan Lu et al. bioRxiv 2019; (Epub) Application of non-invasive low-intensity pulsed electric field with thermal cycling-hyperthermia for synergistically enhanced anticancer effect of chlorogenic acid on PANC-1 cells.
Application : WB
Reactivity : Human
Li H et al. Cells 2019; 8 (9) PFN2a Suppresses C2C12 Myogenic Development by Inhibiting Proliferation and Promoting Apoptosis via the p53 Pathway.
Application : WB
Reactivity : Human
Chen KY et al. PLoS One 2019; 14 (7):e0219317 Resveratrol induced premature senescence and inhibited epithelial-mesenchymal transition of cancer cells via induction of tumor suppressor Rad9.
Application : WB
Reactivity : Human
Tan HW et al. Cells 2019; 8 (8) Lasting DNA Damage and Aberrant DNA Repair Gene Expression Profile Are Associated with Post-Chronic Cadmium Exposure in Human Bronchial Epithelial Cells.
Application : WB
Reactivity : Human
Liu T et al. Neoplasia 2019; 21 (6):557-570 Epigenetically Down-Regulated Acetyltransferase PCAF Increases the Resistance of Colorectal Cancer to 5-Fluorouracil.
Application : IP
Reactivity : Human
Zhang K et al. Mol Cancer 2018; 17 (1):153 Targeting histone methyltransferase G9a inhibits growth and Wnt signaling pathway by epigenetically regulating HP1α and APC2 gene expression in non-small cell lung cancer.
Application : WB
Reactivity : Human
Ying Zhang et al. Oncology Letters 2018; (Epub) Apoptosis of mouse myeloma cells induced by curcumin via the Notch3-p53 signaling axis.
Application : WB
Reactivity : Mouse
Briest F et al. Neuroendocrinology 2017; (Epub) Mechanisms of targeting the MDM2-p53-FOXM1 axis in well-differentiated intestinal neuroendocrine tumors.
Application : WB
Reactivity : Human
Phang CW et al. Pharmacognosy Magazine 2017; 13 (50):321-328 Induction of apoptosis and cell cycle arrest by flavokawain C on HT-29 human colon adenocarcinoma via enhancement of reactive oxygen species generation, upregulation of p21, p27, and Gadd153, and inactivation of inhibitor of apoptosis proteins.
Application : WB
Reactivity : Human
Chang TM et al. Evidence-Based Complementary and Alternative Medicine 2017; 2017:8325049 Extract from Periostracum cicadae Inhibits Oxidative Stress and Inflammation Induced by Ultraviolet B Irradiation on HaCaT Keratinocytes.
Application : WB
Reactivity : Human
Samuel J et al. Cancer Res 2016; Posttranscriptional upregulation of p53 by reactive oxygen species in chronic lymphocytic leukemia.
Application : ICC/IF
Reactivity : Human
Constantinescu AA et al. Mol Cell Biochem 2016; 418 (43102):91-102 Differential influence of * and * on mitochondrial-dependent signaling for apoptosis in pancreatic cells.
Application : WB
Reactivity : Human
Liu MK et al. Int J Mol Sci 2016; 17 (6) Topoisomerase II Inhibitors Can Enhance Baculovirus-Mediated Gene Expression in Mammalian Cells through the DNA Damage Response.
Application : WB
Reactivity : Human
Lee JH et al. PLoS One 2014; 9 (6):e97969 Direct Activation of ATM by Resveratrol under Oxidizing Conditions.
Application : WB
Reactivity : Human
Clone Reference
Jianyuan Luo et al. Cell 2001; 107:137-148 Negative control of p53 by Sir2alpha promotes cell survival under stress.
Application : ICC/IF
Reactivity : Human
Marchenko ND et al. J Biol Chem. 2000; 275:16202-12. Death signal-induced localization of p53 protein to mitochondria. A potential role in apoptotic signaling.
Application : FACS
Reactivity : Human
Vojt?sek B et al. Br J Cancer. 1993; 67:1254-8. Comparison between p53 staining in tissue sections and p53 proteins levels measured by an ELISA technique.
Application : ELISA
Reactivity : Human
SDS
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Package List Price ($)
$ 399