APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
Assay dependent |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragmemt of human p53.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
tumor protein p53 , BCC7 , BMFS5 , LFS1 , P53 , TRP53
Cellular Localization
Cytoplasm,Nucleus,Endoplasmic reticulum,Mitochondrion matrix
Background
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). [provided by RefSeq, Dec 2016]
Database
Research Area
DATA IMAGES
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GTX638291 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [HL2242] (GTX638291) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638291 ICC/IF Image
p53 antibody [HL2242] detects p53 protein at nucleus by immunofluorescent analysis.Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: p53 stained by p53 antibody [HL2242] (GTX638291) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).
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GTX638291 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [HL2242] (GTX638291) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638291 WB Image
Untreated (–) and treated (+) HCT-116 whole cell extract (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [HL2242] (GTX638291) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638291 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [HL2242] (GTX638291) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638291 IHC-P Image
p53 antibody [HL2242] detects p53 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse testis.p53 stained by p53 antibody [HL2242] (GTX638291) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX638291 IHC-P Image
p53 antibody [HL2242] detects p53 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human oral carcinoma.p53 stained by p53 antibody [HL2242] (GTX638291) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX638291 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [HL2242] (GTX638291) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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REFERENCE
There are currently no references for p53 antibody [HL2242] (GTX638291). Be the first to share your publications with this product.
REVIEW
p53 antibody [HL2242]
Cat. No. GTX638291
Rating
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( Average 5 based on 1 users reviews)
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Application
Western Blot(WB)
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( Average 5 based on 1 users reviews)
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Date :
Anonymous submitted on 29-Oct-2023
Application Tested :
WB
Sample Species :
Ms
Sample :
Neuro2A, ID8-6, ID8-1, ID8-4 cells
Amount used :
30μg
Blocking :
5% non-fat milk in TBST, 25°C, 0.5Hr
Primary Antibody :
1:1000, 4°C, > 16Hr
Figure Note :
The signal was developed with Trident femto ECL, GTX14698.