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p53 antibody [HL2242]

Anti-p53 antibody [HL2242] used in Western Blot (WB). GTX638291
Anti-p53 antibody [HL2242] used in Western Blot (WB). GTX638291
Anti-p53 antibody [HL2242] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638291
Anti-p53 antibody [HL2242] used in Western Blot (WB). GTX638291
Anti-p53 antibody [HL2242] used in Western Blot (WB). GTX638291
Anti-p53 antibody [HL2242] used in Western Blot (WB). GTX638291
Anti-p53 antibody [HL2242] used in IHC (Paraffin sections) (IHC-P). GTX638291
Anti-p53 antibody [HL2242] used in IHC (Paraffin sections) (IHC-P). GTX638291

Cat. No. GTX638291

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL2242

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Cat, Dog
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragmemt of human p53.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tumor protein p53 , BCC7 , BMFS5 , LFS1 , P53 , TRP53

Cellular Localization

Cytoplasm,Nucleus,Endoplasmic reticulum,Mitochondrion matrix

Background

This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). [provided by RefSeq, Dec 2016]

Database

Research Area

DATA IMAGES

Anti-p53 antibody [HL2242] used in Western Blot (WB). GTX638291

GTX638291 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [HL2242] (GTX638291) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-p53 antibody [HL2242] used in Western Blot (WB). GTX638291

GTX638291 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [HL2242] (GTX638291) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-p53 antibody [HL2242] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638291

GTX638291 ICC/IF Image

p53 antibody [HL2242] detects p53 protein at nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: p53 stained by p53 antibody [HL2242] (GTX638291) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-p53 antibody [HL2242] used in Western Blot (WB). GTX638291

GTX638291 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [HL2242] (GTX638291) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-p53 antibody [HL2242] used in Western Blot (WB). GTX638291

GTX638291 WB Image

Untreated (–) and treated (+) HCT-116 whole cell extract (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [HL2242] (GTX638291) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-p53 antibody [HL2242] used in Western Blot (WB). GTX638291

GTX638291 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [HL2242] (GTX638291) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-p53 antibody [HL2242] used in IHC (Paraffin sections) (IHC-P). GTX638291

GTX638291 IHC-P Image

p53 antibody [HL2242] detects p53 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
p53 stained by p53 antibody [HL2242] (GTX638291) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-p53 antibody [HL2242] used in IHC (Paraffin sections) (IHC-P). GTX638291

GTX638291 IHC-P Image

p53 antibody [HL2242] detects p53 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human oral carcinoma.
p53 stained by p53 antibody [HL2242] (GTX638291) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

There are currently no references for p53 antibody [HL2242] (GTX638291). Be the first to share your publications with this product.

REVIEW

p53 antibody [HL2242] Cat. No. GTX638291

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 29-Oct-2023
Score :
Application Tested : WB
Sample Species : Ms
Sample : Neuro2A, ID8-6, ID8-1, ID8-4 cells
Amount used : 30μg
Blocking : 5% non-fat milk in TBST, 25°C, 0.5Hr
Primary Antibody : 1:1000, 4°C, > 16Hr
Figure Note : The signal was developed with Trident femto ECL, GTX14698.
Package List Price ($)
$ 399
$ 169
Custom Conjugations