Untreated (–) and treated (+) zebrafish embryos extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [HL2699] (GTX639450) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.