APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
Assay dependent |
Assay dependent |
1:100-1:1000 |
Assay dependent |
Assay dependent |
Assay dependent |
Assay dependent |
Assay dependent |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
HBL100 , HCT116 , 293T , A431
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.42 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Gel-purified fusion protein containing the coding region of human p53 expressed in E. coli.
Purification
Protein G purified
From tissue culture supernatant
Conjugation
Unconjugated
RRID
AB_372643
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
tumor protein p53 , BCC7 , BMFS5 , LFS1 , P53 , TRP53
Cellular Localization
Cytoplasm , Nucleus , Endoplasmic reticulum , Mitochondrion matrix
Background
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). [provided by RefSeq, Dec 2016]
Database
Research Area
DATA IMAGES
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GTX70216 IP Image
Immunoprecipitation of p53 protein. HCT116 lysates with 30uM cisplatin treatment for 24 hours were subjected to immunoprecipitation using (B) normal mouse IgG or (C) 2.5 ug of anti-p53 antibody (GTX70216). (A) Input, 20ug of HCT116 lysate. The precipitated protein was detected by GTX70216 diluted at 1:10000. EasyBlot anti-Mouse IgG Kit (GTX225857-01) was used in Western blot.
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GTX70216 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [Pab1801] (GTX70216) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
The observed expression level is based on the publication: PMID: 23696801
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GTX70216 ChIP assay Image
p53 antibody immunoprecipitates p53 protein-DNA complex in ChIP experiments. ChIP Sample: HCT116 whole cell lysate/extract treated with CPT 500nM for 6hr A. 5 μg preimmune mouse IgG B. 5 μg of p53 antibody (GTX70216) The precipitated DNA was detected by PCR with primer set targeting to p21 promoter.
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GTX70216 WB Image
Sample (30 μg of whole cell lysate) A: HCT116 cells with mock treatment for 24 hr B: HCT116 cells with 30 μM cisplatin treatment for 24 hr 10% SDS PAGE GTX70216 diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX70216 IHC-P Image
p53 antibody [Pab1801] detects p53 protein at nucleus in NCl-H1299 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded NCl-H1299 xenograft. p53 antibody [Pab1801] (GTX70216) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX70216 WB Image
The image shows a Western blot for GTX70216 , testing the threshold detection of the p53 antibody. The lanes are as follows:. Lane 1 - 20ug of A549 lysate, lane 2 - 10ug of A549 lysate, lane 3 - 5ug of A549 lysate, lane 4 - 2.5ug of A549 lysate, lane
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GTX70216 ICC/IF Image
p53 antibody [Pab1801] detects p53 protein at nucleus by immunofluorescent analysis.Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: p53 stained by p53 antibody [Pab1801] (GTX70216) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
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GTX70216 WB Image
The data was published in the journal Int J Mol Sci in 2019. PMID: 30678307
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REFERENCE
REVIEW
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