p53 antibody [Pab1801]

Name: p53 antibody [Pab1801]
Brand: GeneTex
SKU: GTX70216
Price: 329.00 USD
Rating: 5 (1 reviews)

Anti-p53 antibody [Pab1801] used in Immunoprecipitation (IP). GTX70216

Anti-p53 antibody [Pab1801] used in IHC (Paraffin sections) (IHC-P). GTX70216

Anti-p53 antibody [Pab1801] used in Western Blot (WB). GTX70216

Anti-p53 antibody [Pab1801] used in ChIP assay (ChIP assay). GTX70216

Anti-p53 antibody [Pab1801] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70216

Cat No. GTX70216

Reference 2

Host	Mouse
Clonality	Monoclonal
Clone Name	Pab1801
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ELISA, ChIP assay, RIA
Reactivity	Human
Package	100 μl ($329)

See all p53 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	Assay dependent
ICC/IF	Assay dependent
IHC-P	1:100-1:1000
IHC-Fr	Assay dependent
FACS	Assay dependent
IP	Assay dependent
ELISA	Assay dependent
ChIP assay	Assay dependent
RIA	Assay dependent

Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

HBL100 , HCT116 , 293T , A431

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Gel-purified fusion protein containing the coding region of human p53 expressed in E. coli.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

RRID

AB_372643

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tumor protein p53 , BCC7 , BMFS5 , LFS1 , P53 , TRP53

Cellular Localization

Cytoplasm , Nucleus , Endoplasmic reticulum , Mitochondrion matrix

Background

This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). [provided by RefSeq, Dec 2016]

Database

Gene ID: 7157 TP53
UniProt: P04637 TP53

Research Area

DATA IMAGES

GTX70216 IP Image

Immunoprecipitation of p53 protein. HCT116 lysates with 30uM cisplatin treatment for 24 hours were subjected to immunoprecipitation using (B) normal mouse IgG or (C) 2.5 ug of anti-p53 antibody (GTX70216). (A) Input, 20ug of HCT116 lysate. The precipitated protein was detected by GTX70216 diluted at 1:10000. EasyBlot anti-Mouse IgG Kit (GTX225857-01) was used in Western blot.

GTX70216 IHC-P Image

p53 antibody [Pab1801] detects p53 protein at nucleus in NCl-H1299 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded NCl-H1299 xenograft.
p53 antibody [Pab1801] (GTX70216) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX70216 WB Image

Sample (30 μg of whole cell lysate)
A: HCT116 cells with mock treatment for 24 hr
B: HCT116 cells with 30 μM cisplatin treatment for 24 hr
10% SDS PAGE
GTX70216 diluted at 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX70216 ChIP assay Image

p53 antibody immunoprecipitates p53 protein-DNA complex in ChIP experiments. ChIP Sample: HCT116 whole cell lysate/extract treated with CPT 500nM for 6hr
A. 5 μg preimmune mouse IgG
B. 5 μg of p53 antibody (GTX70216)
The precipitated DNA was detected by PCR with primer set targeting to p21 promoter.

GTX70216 WB Image

The image shows a Western blot for GTX70216 , testing the threshold detection of the p53 antibody. The lanes are as follows:.

Lane 1 - 20ug of A549 lysate, lane 2 - 10ug of A549 lysate, lane 3 - 5ug of A549 lysate, lane 4 - 2.5ug of A549 lysate, lane

GTX70216 ICC/IF Image

p53 antibody [Pab1801] detects p53 protein at nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: p53 stained by p53 antibody [Pab1801] (GTX70216) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.

GTX70216 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [Pab1801] (GTX70216) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
The observed expression level is based on the publication: PMID: 23696801

REFERENCE

Application Reference

Chu CW et al. Int J Mol Sci 2019; 20 (3) Thioridazine Enhances P62-Mediated Autophagy and Apoptosis Through Wnt/β-Catenin Signaling Pathway in Glioma Cells.

Application : WB

Reactivity : Human

Yamamura S et al. Mol Cancer Ther 2010; 9 (6) : 1680-7 Oncogenic functions of secreted Frizzled-related protein 2 in human renal cancer.

Application : WB

Reactivity : Human

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