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p53 antibody [Pab1801]

Anti-p53 antibody [Pab1801] used in Immunoprecipitation (IP). GTX70216
Anti-p53 antibody [Pab1801] used in Western Blot (WB). GTX70216
Anti-p53 antibody [Pab1801] used in ChIP assay (ChIP assay). GTX70216
Anti-p53 antibody [Pab1801] used in Western Blot (WB). GTX70216
Anti-p53 antibody [Pab1801] used in IHC (Paraffin sections) (IHC-P). GTX70216
Anti-p53 antibody [Pab1801] used in Western Blot (WB). GTX70216
Anti-p53 antibody [Pab1801] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70216

Cat No. GTX70216

Host

Mouse

Clonality

Monoclonal

Clone Name

Pab1801

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ELISA, ChIP assay, RIA

Reactivity

Human
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
FACS Assay dependent
IP Assay dependent
ELISA Assay dependent
ChIP assay Assay dependent
RIA Assay dependent
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

HBL100 , HCT116 , 293T , A431

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Gel-purified fusion protein containing the coding region of human p53 expressed in E. coli.

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Tumor Protein P53 , Bcc7 , Lfs1 , P53 , Trp53 , Tp53

Cellular Localization

Cytoplasmic and Nuclear

Background

PAb1801 specifically recognizes human p53 protein, a tumor suppressor gene that is mutated in a wide variety of sporadic and familial cancers. p53, a nuclear protein, mediates cell cycle arrest in response to DNA damage and therefore is important in the maintenance of genomic stability. Recent results indicate that an enhanced phosphorylation of p53 by ATM in response to DNA damage on SER15 is a mutation which reduces the ability of p53 to arrest cell growth.

Database

Research Area

DATA IMAGES

Anti-p53 antibody [Pab1801] used in Immunoprecipitation (IP). GTX70216

GTX70216 IP Image

Immunoprecipitation of p53 protein. HCT116 lysates with 30uM cisplatin treatment for 24 hours were subjected to immunoprecipitation using (B) normal mouse IgG or (C) 2.5 ug of anti-p53 antibody (GTX70216). (A) Input, 20ug of HCT116 lysate. The precipitated protein was detected by GTX70216 diluted at 1:10000. EasyBlot anti-Mouse IgG Kit (GTX225857-01) was used in Western blot.

Anti-p53 antibody [Pab1801] used in Western Blot (WB). GTX70216

GTX70216 WB Image

Sample (30 μg of whole cell lysate)
A: HCT116 cells with mock treatment for 24 hr
B: HCT116 cells with 30 μM cisplatin treatment for 24 hr
10% SDS PAGE
GTX70216 diluted at 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-p53 antibody [Pab1801] used in ChIP assay (ChIP assay). GTX70216

GTX70216 ChIP assay Image

p53 antibody immunoprecipitates p53 protein-DNA complex in ChIP experiments. ChIP Sample: HCT116 whole cell lysate/extract treated with CPT 500nM for 6hr
A. 5 μg preimmune mouse IgG
B. 5 μg of p53 antibody (GTX70216)
The precipitated DNA was detected by PCR with primer set targeting to p21 promoter.

Anti-p53 antibody [Pab1801] used in Western Blot (WB). GTX70216

GTX70216 WB Image

The image shows a Western blot for GTX70216 , testing the threshold detection of the p53 antibody. The lanes are as follows:.

Lane 1 - 20ug of A549 lysate, lane 2 - 10ug of A549 lysate, lane 3 - 5ug of A549 lysate, lane 4 - 2.5ug of A549 lysate, lane

Anti-p53 antibody [Pab1801] used in IHC (Paraffin sections) (IHC-P). GTX70216

GTX70216 IHC-P Image

p53 antibody [Pab1801] detects p53 protein at nucleus in NCl-H1299 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded NCl-H1299 xenograft.
p53 antibody [Pab1801] (GTX70216) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-p53 antibody [Pab1801] used in Western Blot (WB). GTX70216

GTX70216 WB Image

Sample (30 μg of whole cell lysate)
A: 293T
B: A431
10% SDS PAGE
GTX70216 diluted at 1:10000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-p53 antibody [Pab1801] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70216

GTX70216 ICC/IF Image

p53 antibody [Pab1801] detects p53 protein at nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: p53 stained by p53 antibody [Pab1801] (GTX70216) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.

REFERENCE

REVIEW

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SDS
PBS.pdf