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p53 antibody [Pab240]

Anti-p53 antibody [Pab240] used in Western Blot (WB). GTX70218
Anti-p53 antibody [Pab240] used in Western Blot (WB). GTX70218
Anti-p53 antibody [Pab240] used in IHC (Paraffin sections) (IHC-P). GTX70218

Cat No. GTX70218

Host

Mouse

Clonality

Monoclonal

Clone Name

Pab240

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ELISA

Reactivity

Human, Mouse, Dog
Package
100 μl ($329)

APPLICATION

Application Note

For IHC: Use at a dilution of 1:250 - 1:500. Extensive washing may be necessary. This product does not require protein digestion or microwave antigen retrieval prior to staining. For IP: Use at 10μg/mg of lysate. By immunoprecipitation, this antibody reacts with only MUTANT p53protein under NON-DENATURING conditions. For WB: Use at a dilution of 1:2,000. Predicted molecular weight: 53 kDa. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

44 kDa. ( Note )

Positive Control

HBL100 , HCT116 , 293T , A431

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Gel-purified p53 containing p53 amino acids 14-389 (derived from the pSV53C p53 cDNA clone).

Purification

Protein G purified
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Tumor Protein P53 , Bcc7 , Lfs1 , P53 , Trp53 , Tp53

Background

PAb240 specifically recognizes human p53 protein, a tumor suppressor gene that is mutated in a wide variety of sporadic and familial cancers. p53, a nuclear protein, mediates cell cycle arrest in response to DNA damage and therefore is important in the maintenance of genomic stability. Recent results indicate that an enhanced phosphorylation of p53 by ATM in response to DNA damage on SER15 is a mutation which reduces the ability of p53 to arrest cell growth.

Database

Research Area

DATA IMAGES

Anti-p53 antibody [Pab240] used in Western Blot (WB). GTX70218

GTX70218 WB Image

Sample (30 μg of whole cell lysate)
A: 293T
B: A431
10% SDS PAGE
GTX70218 diluted at 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-p53 antibody [Pab240] used in Western Blot (WB). GTX70218

GTX70218 WB Image

Sample (30 μg of whole cell lysate)
A: HCT116 cells with mock treatment for 24 hr
B: HCT116 cells with 30 μM cisplatin treatment for 24 hr
10% SDS PAGE
GTX70218 diluted at 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-p53 antibody [Pab240] used in IHC (Paraffin sections) (IHC-P). GTX70218

GTX70218 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using p53(GTX70218) antibody at 1:200 dilution.

REFERENCE

REVIEW

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SDS
PBS.pdf