p53 antibody [TRP/817]

Cat No. GTX34935

Host	Mouse
Clonality	Monoclonal
Clone Name	TRP/817
Isotype	IgG2b
Application	WB, IHC-P
Reactivity	Human
Package	100 μg ($469)

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Datasheet
Document

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1-2μg/ml
IHC-P	1-2μg/ml for 30 minutes at RT
Note : IHC-P Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Product Note

Recognizes a 53kDa protein, which is identified as p53 suppressor gene product. It reacts with the mutant as well as the wild form of p53 protein.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full-length human TP53 protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

BCC7 , LFS1 , P53 , TP53 , TRP53 , tumor protein p53 , p53

Cellular Localization

Cytoplasm,Nucleus,PML body,Endoplasmic reticulum,Mitochondrion matrix

Background

This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). [provided by RefSeq, Dec 2016]

Database

Gene ID: 7157 TP53
UniProt: P04637 TP53

Research Area

DATA IMAGES

GTX34935 WB Image

WB analysis of HeLa cell lysate using GTX34935 p53 antibody [TRP/817].

GTX34935 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX34935 p53 antibody [TRP/817].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μg

$ 469

Qty