GeneTex
United States (US)

p53 antibody [X77]

p53-antibody-X77_WB_GTX16465-1_18121411_748.jpg

Cat No. GTX16465

Host Mouse
Clonality Monoclonal
Clone Name X77
Isotype IgG1
Application WB, IP, ELISA
Reactivity Human, Mouse, Xenopus laevis
APPLICATION

Application Note

ELISA: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at a concentration of 1 - 10 μg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa). Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

44 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

Preservative: 0.08% Sodium Azide. Constituents: PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Xenopus laevis

Immunogen

Full length protein (Xenopus laevis).

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tumor Protein P53,Bcc7,Lfs1,P53,Trp53,Tp53

Cellular Localization

Cytoplasmic and Nuclear

Background

p53 plays a major role in the cellular response to DNA damage and other genomic aberrations. The activation of p53 can lead to either cell cycle arrest and DNA repair, or apoptosis. p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro.

Database

Research Area

DATA IMAGES
p53-antibody-X77_WB_GTX16465-1_18121411_748.jpg

GTX16465 WB Image

Western blot analysis using Manti-p53 cln. X77 antibody at 10 µg/ml on HCT116 cell lysate (1), HCT116 cell lysate activated with adriamycin (2), p21-/- cell lysate (3), P21-/- cell lysate activated with ADR (4) and p53-/- activated with ADR. ADR activates p53 in cells. Also shown is a graphic representation of the epitope location.

Package List Price ($)
$ 329