GeneTex
United States (US)

p53 antibody

KO/KD Orthogonal
GTX102965_IHC_19011506_983.jpg
GTX102965_39981_IFA_2_19011506_609.jpg
GTX102965_40331_20160623_WB_shRNA_watermark_19011506_312.jpg
GTX102965_39981_ChIP_19011506_899.jpg
GTX102965_39981_IFA_19011506_762.jpg
GTX102965_40331_WB_19011506_809.jpg
GTX102965_39981_WB_HCT116 cisplatin_19011720_366.jpg
GTX102965_39981_IP_19011506_293.jpg

Cat No. GTX102965

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP, ChIP assay
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

293T , A431 , HCT116 , HCT116 24hr cisplatin 30uM , *HUVEC

Predict Reactivity

Rat, Rabbit, Sheep, Bovine, Cat, Dog, Guinea pig, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human p53. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tumor Protein P53,Bcc7,Lfs1,P53,Trp53,Tp53

Cellular Localization

Cytoplasm , Nucleus , Endoplasmic reticulum

Background

This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of this gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome. Multiple p53 variants due to alternative promoters and multiple alternative splicing have been found. These variants encode distinct isoforms, which can regulate p53 transcriptional activity. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX102965_IHC_19011506_983.jpg

GTX102965 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using p53(GTX102965) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102965_39981_IFA_2_19011506_609.jpg

GTX102965 ICC/IF Image

p53 antibody detects p53 protein at nucleus by immunofluorescent analysis.
Samples: HCT 116 cells mock (left) and treated with 30 μM Cisplatin for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: p53 protein stained by p53 antibody (GTX102965) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX102965_40331_20160623_WB_shRNA_watermark_19011506_312.jpg

GTX102965 WB Image

KO/KD

Non-transfected (–) and transfected (+) HCT116 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody (GTX102965) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102965_39981_ChIP_19011506_899.jpg

GTX102965 ChIP assay Image

p53 antibody immunoprecipitates p53 protein-DNA complex in ChIP experiments. ChIP Sample: HCT116 whole cell lysate/extract treated with CPT 500nM for 6hr
A. 5 μg preimmune rabbit IgG
B. 5 μg of p53 antibody (GTX102965)
The precipitated DNA was detected by PCR with primer set targeting to p21 promoter.

GTX102965_39981_IFA_19011506_762.jpg

GTX102965 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using p53(GTX102965) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

GTX102965_40331_WB_19011506_809.jpg

GTX102965 WB Image

Sample (30 µg of whole cell lysate)
A: A431 (GTX27909)
10% SDS PAGE
GTX102965 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102965_39981_WB_HCT116 cisplatin_19011720_366.jpg

GTX102965 WB Image

Orthogonal

Sample (30 µg of whole cell lysate)
A: HCT116 cells with mock treatment for 24 hr
B: HCT116 cells with 30 µM cisplatin treatment for 24 hr
10% SDS PAGE
GTX102965 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102965_39981_IP_19011506_293.jpg

GTX102965 IP Image

Immunoprecipitation of p53 protein. HCT116 lysates with 30uM cisplatin treatment for 24 hours were subjected to immunoprecipitation using (B) normal rabbit IgG or (C) 2.5 ug of anti-p53 antibody (GTX102965). (A) Input, 20ug of HCT116 lysates. The precipitated protein was detected by GTX102965 diluted at 1:10000. EasyBlot anti-Rabbit IgG Kit (GTX225856-01) was used in Western blot.

REFERENCE
REVIEW

p53 antibody Cat No. GTX102965

Rating ( Average 4.5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 4.5 based on 2 users reviews)
Date : Anonymous submitted on 24-May-2018
Score :
Application Tested : WB
Sample Species : Ms
Sample : RGC-5 cells
Amount used : 40 μg
Blocking : 5 % milk, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, 48Hr
Date : Anonymous submitted on 28-Oct-2011
Score :
Application Tested : WB
Sample Species : Hu
Sample : HUVEC cells
Amount used : 45μg
Blocking : 5% skin-milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, overnightHr
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169