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p53 antibody

Orthogonal
Anti-p53 antibody used in IHC (Whole mount) (IHC-Wm). GTX128135
Anti-p53 antibody used in Western Blot (WB). GTX128135

Cat. No. GTX128135

Reference 19
3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Wm

Reactivity

 Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all zebrafish p53 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
IHC-Wm 1:100-1:500
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

3dpf zebrafish embryos treated with UVC

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.06 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zebrafish

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of zebrafish p53. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2864277

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tumor protein p53 , brp53 , drp53 , etID22686.5 , fb40d06 , p53 , wu:fb40d06 , zgc:111919

Cellular Localization

Cytoplasm , Nucleus

Background

Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of bax and fas antigen expression, or by repression of Bcl-2 expression.

Database

Research Area

DATA IMAGES

Anti-p53 antibody used in IHC (Whole mount) (IHC-Wm). GTX128135

GTX128135 IHC-Wm Image

Tp53 antibody detects Tp53 protein on UV-irradiated whole-mount zebrafish embryos (right panel) by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed zebrafish embryos.
Tp53 antibody (GTX128135) dilution: 1:200.

The UV-irradiated experiment is based on PMID: 20047468

Anti-p53 antibody used in Western Blot (WB). GTX128135

GTX128135 WB Image

Orthogonal

Untreated (–) and treated (+) zebrafish embryos extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody (GTX128135) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Jingzhen Li et al. Nucleic Acids Res 2021; 49 (4) : 2027-2043 Prpf31 is essential for the survival and differentiation of retinal progenitor cells by modulating alternative splicing
Application : WB
Reactivity : Zebrafish
Shoffner A et al. Cell Rep 2020; 32 (9) : 108089 Tp53 Suppression Promotes Cardiomyocyte Proliferation during Zebrafish Heart Regeneration.
Espanola SG et al. Nat Cell Biol 2020; 22 (12) : 1411-1422 Haematopoietic stem cell-dependent Notch transcription is mediated by p53 through the Histone chaperone Supt16h.
Application : WB
Reactivity : Zebrafish
Mohapatra S et al. Cell Death Differ 2020; (Epub) Estrogen and estrogen receptors chauffeur the sex-biased autophagic action in liver.
Application : WB
Lu LF et al. Fish Shellfish Immunol 2020; 102 : 449-459 Ca2+ plays an antiviral role by increasing p53 expression to achieve protection against spring viraemia of carp virus infection.
Application : WB
Reactivity : Zebrafish
Show More +
Submit a Reference

REVIEW

p53 antibody Cat. No. GTX128135

Rating
( Average 5 based on 3 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 2 users reviews)
Anti-p53 antibody used in Western Blot (WB). GTX128135
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Zfsh
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2015.PMID: 26507500
Anti-p53 antibody used in IHC (Paraffin sections) (IHC-P). GTX128135
Date : Anonymous submitted on 25-Dec-2017
Score :
Application Tested : IHC-P
Sample Species : Zfsh
Sample : Eye tumor tissue
Blocking : Normal goat serum, 0.5Hr
Permeabilization /Antigen retrieval : EDTA pH 9.0
Primary Antibody : 1:700, 4°C, 16Hr
Figure Note : Zebrafish p53 mutant eye tumor, p53 nuclear staining, magnification 200x (A) and 400x (B).
Anti-p53 antibody used in IHC (Paraffin sections) (IHC-P). GTX128135
Date : Anonymous submitted on 25-Dec-2017
Score :
Application Tested : IHC-P
Sample Species : Zfsh
Sample : Kidney tissue
Blocking : Normal goat serum, 0.5Hr
Permeabilization /Antigen retrieval : EDTA pH 9.0
Primary Antibody : 1:700, 4°C, 16Hr
Figure Note : Zebrafish p53 mutant kidney, positive for nuclear staining, magnification 40x (A) and 400x (B).
Submit a Review
Application Reference
Jingzhen Li et al. Nucleic Acids Res 2021; 49 (4):2027-2043 Prpf31 is essential for the survival and differentiation of retinal progenitor cells by modulating alternative splicing
Application : WB
Reactivity : Zebrafish
Shoffner A et al. Cell Rep 2020; 32 (9):108089 Tp53 Suppression Promotes Cardiomyocyte Proliferation during Zebrafish Heart Regeneration.
Espanola SG et al. Nat Cell Biol 2020; 22 (12):1411-1422 Haematopoietic stem cell-dependent Notch transcription is mediated by p53 through the Histone chaperone Supt16h.
Application : WB
Reactivity : Zebrafish
Mohapatra S et al. Cell Death Differ 2020; (Epub) Estrogen and estrogen receptors chauffeur the sex-biased autophagic action in liver.
Application : WB
Lu LF et al. Fish Shellfish Immunol 2020; 102:449-459 Ca2+ plays an antiviral role by increasing p53 expression to achieve protection against spring viraemia of carp virus infection.
Application : WB
Reactivity : Zebrafish
Mawed SA et al. Int J Mol Sci 2020; 21 (4) Strategy of Hepatic Metabolic Defects Induced by beclin1 Heterozygosity in Adult Zebrafish.
Application : WB
Reactivity : Zebrafish
Adriana Petrovici et al. J. Mar. Sci. Eng. 2020; 8 (2):73 Toxicity of Deltamethrin to Zebrafish Gonads Revealed by Cellular Biomarkers.
Application : IHC-P
Reactivity : Zebrafish
Caihong Wang et al. Chemosphere 2020; 241 Triclosan-induced liver and brain injury in zebrafish (Danio rerio) via abnormal expression of miR-125 regulated by PKCα/Nrf2/p53 signaling pathways.
Application : IHC-P
Reactivity : Zebrafish
D'Agati G et al. Dis Model Mech 2019; 12 (7) Active receptor tyrosine kinases
Li H et al. J Cell Physiol 2019; (Epub) Mitochondrial proteomics reveals the mechanism of spermatogenic cells apoptosis induced by carbon ion radiation in zebrafish.
Application : WB
Reactivity : Zebrafish
Liu PH et al. Nat Cell Biol 2019; 21 (2):203-213 An IRAK1-PIN1 signalling axis drives intrinsic tumour resistance to radiation therapy.
Application : WB
Reactivity : Zebrafish
Si J et al. Toxicology 2018; (Epub) Effects of ionizing radiation and HLY78 on the zebrafish embryonic developmental toxicity.
Application : WB
Reactivity : Zebrafish
Shtraizent N et al. Elife 2017; 6 MPI depletion enhances O-GlcNAcylation of p53 and suppresses the Warburg effect.
Application : WB
Reactivity : Zebrafish
Zhou R et al. Mutat Res 2016; 793-794:41-50 Effects of Ru(CO)3Cl-glycinate on the developmental toxicities induced by X-ray and carbon-ion irradiation in zebrafish embryos.
Application : WB
Reactivity : Zebrafish
Wei Y et al. Development 2016; (Epub) RNA polymerase III component Rpc9 regulates hematopoietic stem and progenitor cell maintenance in zebrafish.
Application : WB
Reactivity : Zebrafish
Zhang S et al. Invest Ophthalmol Vis Sci 2016; 57 (4):1991-2000 Antiviral Drug * Is a Potent Inhibitor of the Proliferation of Mller Glia-Derived Progenitors During Zebrafish Retinal Regeneration.
Application : WB
Reactivity : Zebrafish
Jing J et al. Sci Rep 2015; 5:15906 A feedback regulatory loop involving p53/miR-200 and * endocrine axis controls embryo size of zebrafish.
Application : WB
Reactivity : Zebrafish
Percival SM et al. Dis Model Mech 2015; 8 (8):941-55 Variations in dysfunction of sister chromatid cohesion in esco2 mutant zebrafish reflect the phenotypic diversity of Roberts syndrome.
Application : WB
Reactivity : Zebrafish
Tsai M et al. Dev Neurobiol 2014; 75 (9):908-26 Modulation of p53 and met expression by kr-ppel-like factor 8 regulates zebrafish cerebellar development.
Application : WB
Reactivity : Zebrafish
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169
Currently in production