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p53 antibody

Anti-p53 antibody used in IHC (Whole mount) (IHC-Wm). GTX128135
Anti-p53 antibody used in Western Blot (WB). GTX128135

Cat. No. GTX128135

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, IHC-Wm

Reactivity

Zebrafish
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
IHC-Wm 1:100-1:500
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

3dpf zebrafish embryos treated with UVC

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.06 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zebrafish

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of zebrafish p53. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2864277

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tumor protein p53 , brp53 , drp53 , etID22686.5 , fb40d06 , p53 , wu:fb40d06 , zgc:111919

Cellular Localization

Cytoplasm , Nucleus

Background

Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of bax and fas antigen expression, or by repression of Bcl-2 expression.

Database

Research Area

DATA IMAGES

Anti-p53 antibody used in IHC (Whole mount) (IHC-Wm). GTX128135

GTX128135 IHC-Wm Image

Tp53 antibody detects Tp53 protein on UV-irradiated whole-mount zebrafish embryos (right panel) by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed zebrafish embryos.
Tp53 antibody (GTX128135) dilution: 1:200.

The UV-irradiated experiment is based on PMID: 20047468

Anti-p53 antibody used in Western Blot (WB). GTX128135

GTX128135 WB Image

Untreated (–) and treated (+) zebrafish embryos extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody (GTX128135) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

p53 antibody Cat. No. GTX128135

Rating ( Average 5 based on 3 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Zfsh
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2015.PMID: 26507500
Date : Anonymous submitted on 25-Dec-2017
Score :
Application Tested : IHC-P
Sample Species : Zfsh
Sample : Eye tumor tissue
Blocking : Normal goat serum, 0.5Hr
Permeabilization /Antigen retrieval : EDTA pH 9.0
Primary Antibody : 1:700, 4°C, 16Hr
Figure Note : Zebrafish p53 mutant eye tumor, p53 nuclear staining, magnification 200x (A) and 400x (B).
Date : Anonymous submitted on 25-Dec-2017
Score :
Application Tested : IHC-P
Sample Species : Zfsh
Sample : Kidney tissue
Blocking : Normal goat serum, 0.5Hr
Permeabilization /Antigen retrieval : EDTA pH 9.0
Primary Antibody : 1:700, 4°C, 16Hr
Figure Note : Zebrafish p53 mutant kidney, positive for nuclear staining, magnification 40x (A) and 400x (B).
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169
Currently in production