GeneTex
United States (US)

p53 antibody

Orthogonal
GTX128135_41150_IHC-Wm_18121410_735.jpg
GTX128135_42039_20160128_WB_Z_UVC_18121410_565.jpg

Cat No. GTX128135

Reference 9
2
Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IHC-Wm
Reactivity Zebrafish
See all zebrafish p53 products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P Assay dependent
IHC-Wm 1:100-1:500
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

3dpf zebrafish embryos treated with UVC
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.06 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zebrafish

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of zebrafish p53. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tumor Protein P53,Brp53,Drp53,Etid22686.5,Fb40D06,P53,Wu:Fb40D06,Zgc:111919,Tp53

Cellular Localization

Cytoplasm , Nucleus

Background

Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of bax and fas antigen expression, or by repression of Bcl-2 expression.

Database

Research Area

DATA IMAGES
GTX128135_41150_IHC-Wm_18121410_735.jpg

GTX128135 IHC-Wm Image

Tp53 antibody detects Tp53 protein on UV-irradiated whole-mount zebrafish embryos (right panel) by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed zebrafish embryos.
Tp53 antibody (GTX128135) dilution: 1:200.

The UV-irradiated experiment is based on PMID: 20047468.

GTX128135_42039_20160128_WB_Z_UVC_18121410_565.jpg

GTX128135 WB Image

Orthogonal

Untreated (–) and treated (+) zebrafish embryos extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody (GTX128135) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
REFERENCE

Application Reference

Liu PH et al. Nat Cell Biol 2019; 21 (2) : 203-213 An IRAK1-PIN1 signalling axis drives intrinsic tumour resistance to radiation therapy.
Application : WB
Reactivity : Zebrafish
Si J et al. Toxicology 2018; (Epub ahead of print) Effects of ionizing radiation and HLY78 on the zebrafish embryonic developmental toxicity.
Application : WB
Reactivity : Zebrafish
Shtraizent N et al. Elife 2017; 6 MPI depletion enhances O-GlcNAcylation of p53 and suppresses the Warburg effect.
Application : WB
Reactivity : Zebrafish
Zhou R et al. Mutat Res 2016; 793-794 : 41-50 Effects of Ru(CO)3Cl-glycinate on the developmental toxicities induced by X-ray and carbon-ion irradiation in zebrafish embryos.
Application : WB
Reactivity : Zebrafish
Wei Y et al. Development 2016; (Epub ahead of print) RNA polymerase III component Rpc9 regulates hematopoietic stem and progenitor cell maintenance in zebrafish.
Application : WB
Reactivity : Zebrafish
Show More +
Submit a Reference
REVIEW

p53 antibody Cat No. GTX128135

Rating
( Average 5 based on 2 users reviews)
Application
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 25-Dec-2017
Score :
Application Tested : IHC-P
Sample Species : Zfsh
Sample : Eye tumor tissue
Blocking : Normal goat serum, 0.5Hr
Permeabilization /Antigen retrieval : EDTA pH 9.0
Primary Antibody : 1:700, 4°C, overnightHr
Figure Note : Zebrafish p53 mutant eye tumor, p53 nuclear staining, magnification 200x (A) and 400x (B).
Date : Anonymous submitted on 25-Dec-2017
Score :
Application Tested : IHC-P
Sample Species : Zfsh
Sample : Kidney tissue
Blocking : Normal goat serum, 0.5Hr
Permeabilization /Antigen retrieval : EDTA pH 9.0
Primary Antibody : 1:700, 4°C, overnightHr
Figure Note : Zebrafish p53 mutant kidney, positive for nuclear staining, magnification 40x (A) and 400x (B).
Submit a Review
Application Reference
Liu PH et al. Nat Cell Biol 2019; 21 (2):203-213 An IRAK1-PIN1 signalling axis drives intrinsic tumour resistance to radiation therapy.
Application : WB
Reactivity : Zebrafish
Si J et al. Toxicology 2018; (Epub ahead of print) Effects of ionizing radiation and HLY78 on the zebrafish embryonic developmental toxicity.
Application : WB
Reactivity : Zebrafish
Shtraizent N et al. Elife 2017; 6 MPI depletion enhances O-GlcNAcylation of p53 and suppresses the Warburg effect.
Application : WB
Reactivity : Zebrafish
Zhou R et al. Mutat Res 2016; 793-794:41-50 Effects of Ru(CO)3Cl-glycinate on the developmental toxicities induced by X-ray and carbon-ion irradiation in zebrafish embryos.
Application : WB
Reactivity : Zebrafish
Wei Y et al. Development 2016; (Epub ahead of print) RNA polymerase III component Rpc9 regulates hematopoietic stem and progenitor cell maintenance in zebrafish.
Application : WB
Reactivity : Zebrafish
Zhang S et al. Invest Ophthalmol Vis Sci 2016; 57 (4):1991-2000 Antiviral Drug Ganciclovir Is a Potent Inhibitor of the Proliferation of Mller Glia-Derived Progenitors During Zebrafish Retinal Regeneration.
Application : WB
Reactivity : Zebrafish
Jing J et al. Sci Rep 2015; 5:15906 A feedback regulatory loop involving p53/miR-200 and growth hormone endocrine axis controls embryo size of zebrafish.
Application : WB
Reactivity : Zebrafish
Percival SM et al. Dis Model Mech 2015; 8 (8):941-55 Variations in dysfunction of sister chromatid cohesion in esco2 mutant zebrafish reflect the phenotypic diversity of Roberts syndrome.
Application : WB
Reactivity : Zebrafish
Tsai M et al. Dev Neurobiol 2014; 75 (9):908-26 Modulation of p53 and met expression by kr-ppel-like factor 8 regulates zebrafish cerebellar development.
Application : WB
Reactivity : Zebrafish
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 389
$ 199