GeneTex
United States (US)

p53 antibody

Orthogonal
GTX128135_41150_IHC-Wm_18121410_735.jpg
GTX128135_42039_20160128_WB_Z_UVC_18121410_565.jpg

Cat No. GTX128135

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IHC-Wm
Reactivity Zebrafish
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P Assay dependent
IHC-Wm 1:100-1:500
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

3dpf zebrafish embryos treated with UVC
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.06mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Zebrafish

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of zebrafish p53. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

tumor suppressor homolog p53 antibody, cellular tumor antigen p53 antibody, etID22686.5 antibody, tumor suppressor p53 antibody, brp53 antibody, drp53 antibody, fb40d06 antibody, p53 antibody, wu:fb40d06 antibody, zgc:111919 antibody, tp53 antibody, tumor protein p53 antibody

Cellular Localization

Cytoplasm (By similarity) , Nucleus

Background

Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of bax and fas antigen expression, or by repression of Bcl-2 expression.

Database

Research Area

DATA IMAGES
GTX128135_41150_IHC-Wm_18121410_735.jpg

GTX128135 IHC-Wm Image

Tp53 antibody detects Tp53 protein on UV-irradiated whole-mount zebrafish embryos (right panel) by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed zebrafish embryos.
Tp53 antibody (GTX128135) dilution: 1:200.

The UV-irradiated experiment is based on PMID: 20047468.

GTX128135_42039_20160128_WB_Z_UVC_18121410_565.jpg

GTX128135 WB Image

Orthogonal

Untreated (–) and treated (+) zebrafish embryos extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody (GTX128135) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW

p53 antibody Cat No. GTX128135

Rating ( Average 5 based on 2 users reviews)
Application
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 25-Dec-2017
Score :
Application Tested : IHC-P
Sample Species : Zfsh
Sample : Eye tumor tissue
Blocking : Normal goat serum, 0.5Hr
Permeabilization /Antigen retrieval : EDTA pH 9.0
Primary Antibody : 1:700, 4°C, overnightHr
Figure Note : Zebrafish p53 mutant eye tumor, p53 nuclear staining, magnification 200x (A) and 400x (B).
Date : Anonymous submitted on 25-Dec-2017
Score :
Application Tested : IHC-P
Sample Species : Zfsh
Sample : Kidney tissue
Blocking : Normal goat serum, 0.5Hr
Permeabilization /Antigen retrieval : EDTA pH 9.0
Primary Antibody : 1:700, 4°C, overnightHr
Figure Note : Zebrafish p53 mutant kidney, positive for nuclear staining, magnification 40x (A) and 400x (B).
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf