GeneTex
United States (US)

p53 antibody

p53-antibody_WB_GTX16121-1_18121411_205.jpg

Cat No. GTX16121

Host Sheep
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IP
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

IHC-P: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at a concentration of 1 - 10 μg/ml. Predicted molecular weight: 53 kDa. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

44 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

Preservative: 0.08% Sodium Azide. Constituents: PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Fusion protein, corresponding to amino acids 1-393 of Human p53.

Purification


Purified by Ammonium Sulfate precipitation.This antibody is provided as a 0.2μm sterile filtered solution.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tumor Protein P53,Bcc7,Lfs1,P53,Trp53,Tp53

Cellular Localization

Cytoplasmic and Nuclear

Background

p53 plays a major role in the cellular response to DNA damage and other genomic aberrations. The activation of p53 can lead to either cell cycle arrest and DNA repair, or apoptosis. p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro.

Database

Research Area

DATA IMAGES
p53-antibody_WB_GTX16121-1_18121411_205.jpg

GTX16121 WB Image

Each lane contains 50µg of total cellular protein separated by 10% SDS-PAGE. (1) Murine mutant p53; (2) murine wild-type p53; (3) wild-type p53 protein from human colorectal carcinoma cells (RKO); (4) elevated levels of human p53 protein following treatment of RKO cells with adriamycin (0.2 µm).

Package List Price ($)
$ 329