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p53 antibody

Anti-p53 antibody used in Western Blot (WB). GTX50438
Anti-p53 antibody used in Western Blot (WB). GTX50438
Anti-p53 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50438
Anti-p53 antibody used in Immunoprecipitation (IP). GTX50438
Anti-p53 antibody used in Western Blot (WB). GTX50438
Anti-p53 antibody used in IHC (Paraffin sections) (IHC-P). GTX50438
Anti-p53 antibody used in IHC (Paraffin sections) (IHC-P). GTX50438
Anti-p53 antibody used in IHC (Paraffin sections) (IHC-P). GTX50438
Anti-p53 antibody used in IHC (Paraffin sections) (IHC-P). GTX50438

Cat No. GTX50438

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
IP Assay dependent
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around aa.13~17 (P-L-S-Q-E) derived from human p53.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tumor protein p53 , BCC7 , BMFS5 , LFS1 , P53 , TRP53

Cellular Localization

Cytoplasm,Nucleus,Endoplasmic reticulum,Mitochondrion matrix

Background

This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). [provided by RefSeq, Dec 2016]

Database

Research Area

DATA IMAGES

Anti-p53 antibody used in Western Blot (WB). GTX50438

GTX50438 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody (GTX50438) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-p53 antibody used in Western Blot (WB). GTX50438

GTX50438 WB Image

WB analysis of extracts from various sample using GTX50438 p53 antibody.
Lane 1 : rat breast tissue
Lane 2 : rat colon tissue
Dilution : 1:1000

Anti-p53 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50438

GTX50438 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50438 p53 antibody.

Anti-p53 antibody used in Immunoprecipitation (IP). GTX50438

GTX50438 IP Image

IP analysis of extracts from 293T cells using GTX50438 p53 antibody.

Anti-p53 antibody used in Western Blot (WB). GTX50438

GTX50438 WB Image

WB analysis of extracts from mouse brain tissue using GTX50438 p53 antibody.

Anti-p53 antibody used in IHC (Paraffin sections) (IHC-P). GTX50438

GTX50438 IHC-P Image

IHC-P analysis of rat breast tissue sections using GTX50438 p53 antibody.

Anti-p53 antibody used in IHC (Paraffin sections) (IHC-P). GTX50438

GTX50438 IHC-P Image

IHC-P analysis of rat breast tissue using GTX50438 p53 antibody.
Dilution : 1:100

Anti-p53 antibody used in IHC (Paraffin sections) (IHC-P). GTX50438

GTX50438 IHC-P Image

IHC-P analysis of rat colon tissue sections using GTX50438 p53 antibody.

Anti-p53 antibody used in IHC (Paraffin sections) (IHC-P). GTX50438

GTX50438 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50438 p53 antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf