Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody (GTX50438) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

ICC/IF analysis of methanol-fixed HeLa cells using GTX50438 p53 antibody.

WB analysis of extracts from mouse brain tissue using GTX50438 p53 antibody.

IP analysis of extracts from 293T cells using GTX50438 p53 antibody.

IHC-P analysis of rat colon tissue sections using GTX50438 p53 antibody.

IHC-P analysis of rat breast tissue using GTX50438 p53 antibody.
Dilution : 1:100

WB analysis of extracts from various sample using GTX50438 p53 antibody.
Lane 1 : rat breast tissue
Lane 2 : rat colon tissue
Dilution : 1:1000

IHC-P analysis of rat breast tissue sections using GTX50438 p53 antibody.

IHC-P analysis of human breast carcinoma tissue using GTX50438 p53 antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide