p53 (mono-methyl Lys372) antibody detects p53 (mono-methyl Lys372) protein at nucleus and endoplasmic reticulum by immunofluorescent analysis.
Sample: Mock and treated HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: p53 (mono-methyl Lys372) stained by p53 (mono-methyl Lys372) antibody (GTX117515) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Untreated (–) and treated (+) HCT-116 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 (mono-methyl Lys372) antibody (GTX117515) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

p53 (mono-methyl Lys372) antibody detects p53 (mono-methyl Lys372) protein at cytoplasm and nucleus in human cervical cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical cancer.
p53 (mono-methyl Lys372) antibody (GTX117515) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using p53 (mono-methyl Lys372)(GTX117515) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.