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p53 (mono-methyl Lys372) antibody

Anti-p53 (mono-methyl Lys372) antibody used in IHC (Paraffin sections) (IHC-P). GTX117515
Anti-p53 (mono-methyl Lys372) antibody used in Western Blot (WB). GTX117515
Anti-p53 (mono-methyl Lys372) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117515
Anti-p53 (mono-methyl Lys372) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117515

Cat No. GTX117515

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Frog, Squirrel
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Positive Control

HCT116 24hr cisplatin 30uM , 48hr cisplatin uM

Predict Reactivity

Sheep, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.43 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human p53 (mono-methyl Lys372). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10617499

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tumor protein p53 , BCC7 , BMFS5 , LFS1 , P53 , TRP53

Cellular Localization

Cytoplasm , Nucleus , Endoplasmic reticulum

Background

This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of this gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome. Multiple p53 variants due to alternative promoters and multiple alternative splicing have been found. These variants encode distinct isoforms, which can regulate p53 transcriptional activity. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-p53 (mono-methyl Lys372) antibody used in IHC (Paraffin sections) (IHC-P). GTX117515

GTX117515 IHC-P Image

p53 (mono-methyl Lys372) antibody detects p53 (mono-methyl Lys372) protein at cytoplasm and nucleus in human cervical cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical cancer.
p53 (mono-methyl Lys372) antibody (GTX117515) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-p53 (mono-methyl Lys372) antibody used in Western Blot (WB). GTX117515

GTX117515 WB Image

Untreated (–) and treated (+) HCT-116 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 (mono-methyl Lys372) antibody (GTX117515) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-p53 (mono-methyl Lys372) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117515

GTX117515 ICC/IF Image

p53 (mono-methyl Lys372) antibody detects p53 (mono-methyl Lys372) protein at nucleus and endoplasmic reticulum by immunofluorescent analysis.
Sample: Mock and treated HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: p53 (mono-methyl Lys372) stained by p53 (mono-methyl Lys372) antibody (GTX117515) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-p53 (mono-methyl Lys372) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117515

GTX117515 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using p53 (mono-methyl Lys372)(GTX117515) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169