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p53 (phospho Ser15) antibody

Anti-p53 (phospho Ser15) antibody used in Western Blot (WB). GTX21431

Cat No. GTX21431

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IP, IHC

Reactivity

Human, Mouse, Rat
Package
50 μg ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
IP Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Product Note

The phospho-p53 (Ser15) antibody detects ~53 kDa phosphorylated p53 at Ser15. It does not react with non-phosphorylated p53 or p53 phosphorylated at other sites.

PROPERTIES

Form

Liquid

Buffer

PBS pH7.2, 30% Glycerol, 0.5% BSA

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic phospho-peptide surrounding amino acid Ser 15 of human p53.

Conjugation

Unconjugated

RRID

AB_367777

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tumor protein p53 , BCC7 , BMFS5 , LFS1 , P53 , TRP53

Cellular Localization

Cytoplasm,Nucleus,Endoplasmic reticulum,Mitochondrion matrix

Background

The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. The activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis. p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro. p53 can apparently be phosphorylated by ATM, ATR, and DNA-PK at Ser15; the phosphorylation impairs the ability of MDM2 to bind p53, promoting both the accumulation and functional activation of p53 in response to DNA damage.

Database

Research Area

DATA IMAGES

Anti-p53 (phospho Ser15) antibody used in Western Blot (WB). GTX21431

GTX21431 WB Image

Western blot analysis of extract from untreated (Lane 1) and UV treated (lane 2) HeLa cells using GTX21431.

REFERENCE

REVIEW

p53 (phospho Ser15) antibody Cat No. GTX21431

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2019. PMID: 31471547
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2019. PMID: 31164689
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319