APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| ~1 μg/ml |
| Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
Filter-sterilized PBS, 50% Glycerol
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
synthetic peptide containing phospho-Ser20
Purification
Purified IgG
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
tumor protein p53 , BCC7 , BMFS5 , LFS1 , P53 , TRP53
Cellular Localization
Cytoplasm,Nucleus,Endoplasmic reticulum,Mitochondrion matrix
Background
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). [provided by RefSeq, Dec 2016]
Database
Research Area
DATA IMAGES
![Anti-p53 (phospho Ser20) antibody [17B6] used in Western Blot (WB). GTX00681](/templates/default/js/lightbox/images/loading.gif "Anti-p53 (phospho Ser20) antibody [17B6] used in Western Blot (WB). GTX00681")
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GTX00681 WB Image
WB analysis of various samples using GTX00681 p53 (phospho Ser20) antibody [17B6] (upper panel) or anti-p53 antibody (lower panel). All samples were immunoprecipitated with anti-Flag antibody and then analyzed by Western blot.
Left : H1299 cells transfected with Flag vector
Middle : H1299 cells transfected with Flag-p53 (wild-type)
Right : H1299 cells transfected with Flag-p53 mutant (Ser20Ala).
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REFERENCE
There are currently no references for p53 (phospho Ser20) antibody [17B6] (GTX00681). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for p53 (phospho Ser20) antibody [17B6] (GTX00681). Be the first to share your experience with this product.
![Anti-p53 (phospho Ser20) antibody [17B6] used in Western Blot (WB). GTX00681](/upload/website/prouct_img/small/GTX00681/GTX00681_20191104_WB_w_23053121_512.webp "Anti-p53 (phospho Ser20) antibody [17B6] used in Western Blot (WB). GTX00681")
![Anti-p53 (phospho Ser20) antibody [17B6] used in Western Blot (WB). GTX00681](/upload/website/prouct_img/normal/GTX00681/GTX00681_20191104_WB_w_23053121_512.webp "Anti-p53 (phospho Ser20) antibody [17B6] used in Western Blot (WB). GTX00681")