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p53 (phospho Ser20) antibody [17B6]

Anti-p53 (phospho Ser20) antibody [17B6] used in Western Blot (WB). GTX00681

Cat No. GTX00681

Host

Mouse

Clonality

Monoclonal

Clone Name

17B6

Isotype

IgG1

Application

WB, ELISA

Reactivity

Human
Package
100 μg ($359)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB ~1 μg/ml
ELISA Assay dependent
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

synthetic peptide containing phospho-Ser20

Purification

Purified IgG

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tumor protein p53 , BCC7 , BMFS5 , LFS1 , P53 , TRP53

Cellular Localization

Cytoplasm,Nucleus,Endoplasmic reticulum,Mitochondrion matrix

Background

This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). [provided by RefSeq, Dec 2016]

Database

Research Area

DATA IMAGES

Anti-p53 (phospho Ser20) antibody [17B6] used in Western Blot (WB). GTX00681

GTX00681 WB Image

WB analysis of various samples using GTX00681 p53 (phospho Ser20) antibody [17B6] (upper panel) or anti-p53 antibody (lower panel). All samples were immunoprecipitated with anti-Flag antibody and then analyzed by Western blot.
Left : H1299 cells transfected with Flag vector
Middle : H1299 cells transfected with Flag-p53 (wild-type)
Right : H1299 cells transfected with Flag-p53 mutant (Ser20Ala).

REFERENCE

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REVIEW

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Package List Price ($)
$ 359