*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
PBS (pH 7.4), 50% glycerol
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Synthetic peptide containing phospho-Ser315 of p53
From tissue culture supernatant
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
tumor protein p53 , BCC7 , BMFS5 , LFS1 , P53 , TRP53
Cytoplasm,Nucleus,Endoplasmic reticulum,Mitochondrion matrix
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). [provided by RefSeq, Dec 2016]
GTX00678 WB Image
WB analysis of various samples using GTX00678 p53 (phospho Ser315) antibody [#18] (upper) or GTX70214 p53 antibody [DO1] (lower).
Lane 1 : untreated MCF cells
Lane 2 : MCF cells treated with nocodazole at 100 ng/ml for 48 hrs
GTX00678 IHC-P Image
IHC-P analysis of Human stomach cancer tissue section using GTX00678 p53 (phospho Ser315) antibody [#18].
GTX00678 ICC/IF Image
ICC/IF analysis of HeLa cells treated with 100 nM Doxorubicin for 24 hrs using GTX00678 p53 (phospho Ser315) antibody [#18].
Dilution : 1:1000 in 1% BSA in PBS, overnight, 4 degree C
Fixation : 4% PFA, overnight
Permeabilization : 0.25% Triton X-100 in PBS for 10 min