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p53 (phospho Ser315) antibody [#18]

Orthogonal
Anti-p53 (phospho Ser315) antibody [#18] used in Western Blot (WB). GTX00678
Anti-p53 (phospho Ser315) antibody [#18] used in IHC (Paraffin sections) (IHC-P). GTX00678
Anti-p53 (phospho Ser315) antibody [#18] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00678

Cat No. GTX00678

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 #18

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P, ELISA

Reactivity

 Human
Package
100 μg ($359)
See all p53 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:2000
ICC/IF Assay dependent
IHC-P Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (pH7.4), 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide containing phospho-Ser315 of p53

Purification

Purified IgG
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tumor protein p53 , BCC7 , BMFS5 , LFS1 , P53 , TRP53

Cellular Localization

Cytoplasm,Nucleus,Endoplasmic reticulum,Mitochondrion matrix

Background

This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). [provided by RefSeq, Dec 2016]

Database

Research Area

DATA IMAGES

Anti-p53 (phospho Ser315) antibody [#18] used in Western Blot (WB). GTX00678

GTX00678 WB Image

Orthogonal

WB analysis of various samples using GTX00678 p53 (phospho Ser315) antibody [#18] (upper) or GTX70214 p53 antibody [DO1] (lower).
Lane 1 : untreated MCF cells
Lane 2 : MCF cells treated with nocodazole at 100 ng/ml for 48 hrs

Anti-p53 (phospho Ser315) antibody [#18] used in IHC (Paraffin sections) (IHC-P). GTX00678

GTX00678 IHC-P Image

IHC-P analysis of Human stomach cancer tissue section using GTX00678 p53 (phospho Ser315) antibody [#18].

Anti-p53 (phospho Ser315) antibody [#18] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00678

GTX00678 ICC/IF Image

ICC/IF analysis of HeLa cells treated with 100 nM Doxorubicin for 24 hrs using GTX00678 p53 (phospho Ser315) antibody [#18].
Dilution : 1:1000 in 1% BSA in PBS, overnight, 4 degree C
Fixation : 4% PFA, overnight
Permeabilization : 0.25% Triton X-100 in PBS for 10 min

REFERENCE

Application Reference

Biswas S et al. Asian Pac J Cancer Prev 2021; 22 (3) : 957-970 Curcumin Rescues Doxorubicin Responsiveness via Regulating Aurora a Signaling Network in Breast Cancer Cells.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 359