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p53 (phospho Ser33) antibody

Anti-p53 (phospho Ser33) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50161
Anti-p53 (phospho Ser33) antibody used in Western Blot (WB). GTX50161
Anti-p53 (phospho Ser33) antibody used in Western Blot (WB). GTX50161

Cat No. GTX50161

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of serine 33 (V-L-S(p)-P-L) derived from human p53.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tumor protein p53 , BCC7 , BMFS5 , LFS1 , P53 , TRP53

Cellular Localization

Cytoplasm,Nucleus,Endoplasmic reticulum,Mitochondrion matrix

Background

This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). [provided by RefSeq, Dec 2016]

Database

Research Area

DATA IMAGES

Anti-p53 (phospho Ser33) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50161

GTX50161 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using GTX50161 p53 (phospho Ser33) antibody.

Anti-p53 (phospho Ser33) antibody used in Western Blot (WB). GTX50161

GTX50161 WB Image

WB analysis of extracts from HT-29 cells using GTX50161 p53 (phospho Ser33) antibody with or without blocking peptide.

Anti-p53 (phospho Ser33) antibody used in Western Blot (WB). GTX50161

GTX50161 WB Image

WB analysis of extracts from HT-29 cells treated with serum or calf intestinal phosphatase (CIP) using GTX50161 p53 (phospho Ser33) antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf