APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:500-1:1000 |
| 1:100-1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide sequence around phosphorylation site of serine 33 (V-L-S(p)-P-L) derived from human p53.
Purification
Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
tumor protein p53 , BCC7 , BMFS5 , LFS1 , P53 , TRP53
Cellular Localization
Cytoplasm,Nucleus,Endoplasmic reticulum,Mitochondrion matrix
Background
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). [provided by RefSeq, Dec 2016]
Database
Research Area
DATA IMAGES
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GTX50161 ICC/IF Image
ICC/IF analysis of methanol-fixed HeLa cells using GTX50161 p53 (phospho Ser33) antibody.
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GTX50161 WB Image
WB analysis of extracts from HT-29 cells using GTX50161 p53 (phospho Ser33) antibody with or without blocking peptide.
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GTX50161 WB Image
WB analysis of extracts from HT-29 cells treated with serum or calf intestinal phosphatase (CIP) using GTX50161 p53 (phospho Ser33) antibody.
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REFERENCE
There are currently no references for p53 (phospho Ser33) antibody (GTX50161). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for p53 (phospho Ser33) antibody (GTX50161). Be the first to share your experience with this product.
![Anti-CA19-9 antibody [GT933] used in IHC (Paraffin sections) (IHC-P). GTX635389](/upload/website/prouct_img/small/GTX635389/GTX635389_43899_20200505_IHC-P_competitor_3_20051723_397.jpg "Anti-CA19-9 antibody [GT933] used in IHC (Paraffin sections) (IHC-P). GTX635389")
