GeneTex
United States (US)

p53 (phospho Ser37) antibody

GTX38640_ICCIF_18121410_531.jpg
GTX38640_WB_18121410_425.jpg

Cat No. GTX38640

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

44 kDa kDa. ( Note )

Specificity/Sensitivity

p53 (phospho-Ser37) antibody detects endogenous levels of p53 only when phosphorylated at serine 37.
PROPERTIES

Form

Liquid

Buffer

In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Concentration

1mg/ml

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human p53 around the phosphorylation site of serine 37 (L -P-SP-Q-A).

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

BCC7 Antibody , TRP53 Antibody , LFS1 Antibody , tumor protein p53 Antibody , P53 Antibody , TP53 Antibody

Background

This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons (PMIDs: 12032546, 20937277). [provided by RefSeq, Feb 2013]
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Database

Research Area

DATA IMAGES
GTX38640_ICCIF_18121410_531.jpg

GTX38640 ICC/IF Image

ICC/IF analysis of methanol-fixed HeLa cells using p53 (phospho Ser37) antibody.

GTX38640_WB_18121410_425.jpg

GTX38640 WB Image

WB analysis of extract from HT-29 cells, untreated or treated with Doxorubicin (1mM, 30 min), using p53 antibody (Lane 1 and 2) and p53 (phospho Ser37) antibody (Lane 3 and 4).