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p53 (phospho Ser46) antibody [#36]

Comparison Orthogonal
Anti-p53 (phospho Ser46) antibody [#36] used in IHC (Paraffin sections) (IHC-P). GTX00680
Anti-p53 (phospho Ser46) antibody [#36] used in Western Blot (WB). GTX00680
Anti-p53 (phospho Ser46) antibody [#36] used in Western Blot (WB). GTX00680
Anti-p53 (phospho Ser46) antibody [#36] used in Western Blot (WB). GTX00680

Cat. No. GTX00680

Reference 3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 #36

Isotype

 IgG1

Application

 WB, IHC-P, ELISA

Reactivity

 Human
Package
100 μg ($399)
See all p53 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:2000
IHC-P Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide containing phospho-Ser46 of p53

Purification

Purified IgG
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tumor protein p53 , BCC7 , BMFS5 , LFS1 , P53 , TRP53

Cellular Localization

Cytoplasm,Nucleus,Endoplasmic reticulum,Mitochondrion matrix

Background

This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). [provided by RefSeq, Dec 2016]

Database

Research Area

DATA IMAGES

Anti-p53 (phospho Ser46) antibody [#36] used in IHC (Paraffin sections) (IHC-P). GTX00680

GTX00680 IHC-P Image

IHC-P analysis of Human stomach cancer tissue section using GTX00680 p53 (phospho Ser46) antibody [#36].

Anti-p53 (phospho Ser46) antibody [#36] used in Western Blot (WB). GTX00680

GTX00680 WB Image

Comparison Orthogonal

WB analysis of MOLT-4 cells treated with/without adriamycin for 24 hrs using GTX00680 p53 (phospho Ser46) antibody [#36].
Left : GTX00680
Right : the competitor's p53 phospho-Ser46 antibody

Anti-p53 (phospho Ser46) antibody [#36] used in Western Blot (WB). GTX00680

GTX00680 WB Image

Orthogonal

WB analysis of various samples using GTX00680 p53 (phospho Ser46) antibody [#36] (upper panel) or anti-p53 antibody (lower panel).
Samples : U2OS cells were irradiated by X-ray (10 Gy) for 0, 0.5, 1, 2, and 4 hrs

Anti-p53 (phospho Ser46) antibody [#36] used in Western Blot (WB). GTX00680

GTX00680 WB Image

Orthogonal

WB analysis of various samples using GTX00680 p53 (phospho Ser46) antibody [#36] (upper panel) or anti-p53 antibody (lower panel).
Samples : MCF-7 cells were irradiated by UV (20 J/m2) for 0, 1, 2, 4, and 8 hrs

REFERENCE

Clone Reference

Taira N et al. J Biol Chem.? 2010; 285 (7):4909-19 ATM augments nuclear stabilization of DYRK2 by inhibiting MDM2 in the apoptotic response to DNA damage.
Application : WB
Reactivity : Human
Kodama M et al. Mol Cell Biol. 2010; 30 (7):1620-33 Requirement of ATM for rapid p53 phosphorylation at Ser46 without Ser/Thr-Gln sequences.
Application : WB
Reactivity : Human
Taira N et al. Mol Cell. 2007; 25 (5):725-38 DYRK2 is targeted to the nucleus and controls p53 via Ser46 phosphorylation in the apoptotic response to DNA damage.
Application : WB
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 399