APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
293T (0.5 μM doxorubicin treatment for 24 hr)
Predict Reactivity
Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide surrounding phospho Ser46 of human p53. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2888418
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
tumor protein p53 , BCC7 , BMFS5 , LFS1 , P53 , TRP53
Cellular Localization
Cytoplasm , Nucleus , Endoplasmic reticulum
Background
This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of this gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome. Multiple p53 variants due to alternative promoters and multiple alternative splicing have been found. These variants encode distinct isoforms, which can regulate p53 transcriptional activity. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX634168 IHC-P Image
p53 (phospho Ser46) antibody [GT1018] detects p53 (phospho Ser46) protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human pancreatic cancer.p53 (phospho Ser46) stained by p53 (phospho Ser46) antibody [GT1018] (GTX634168) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX634168 WB Image
Untreated (–) and treated (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 (phospho Ser46) antibody [GT1018] (GTX634168) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX634168 IHC-P Image
p53 (phospho Ser46) antibody [GT1018] detects p53 (phospho Ser46) protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human colon cancer.p53 (phospho Ser46) stained by p53 (phospho Ser46) antibody [GT1018] (GTX634168) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX634168 IHC-P Image
p53 (phospho Ser46) antibody [GT1018] detects p53 (phospho Ser46) protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human ovarian cancer.p53 (phospho Ser46) stained by p53 (phospho Ser46) antibody [GT1018] (GTX634168) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX634168 ICC/IF Image
p53 (phospho Ser46) antibody [GT1018] detects p53 (phospho Ser46) protein at nucleus by immunofluorescent analysis. Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: p53 (phospho Ser46) protein stained by p53 (phospho Ser46) antibody [GT1018] (GTX634168) diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
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GTX634168 IHC-P Image
p53 (phospho Ser46) antibody [GT1018] detects p53 (phospho Ser46) protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human pancreatic cancer.p53 (phospho Ser46) stained by p53 (phospho Ser46) antibody [GT1018] (GTX634168) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
REVIEW
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