APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:1000-1:2000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
1x TBS (pH7.4), 1% BSA, 40% Glycerol
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein
Purification
Protein A purified
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
tumor protein p53 , BCC7 , LFS1 , P53 , TP53 , TRP53 , p53
Cellular Localization
Cytoplasm,Nucleus,PML body,Endoplasmic reticulum,Mitochondrion matrix
Background
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). [provided by RefSeq, Dec 2016]
Database
Research Area
DATA IMAGES
![Anti-p53 (phospho Thr55) antibody [ST0439] used in Western Blot (WB). GTX01030](/templates/default/js/lightbox/images/loading.gif "Anti-p53 (phospho Thr55) antibody [ST0439] used in Western Blot (WB). GTX01030")
|
GTX01030 WB Image
Untreated (–) and treated (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 (phospho Thr55) antibody [ST0439] (GTX01030) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
|
REFERENCE
There are currently no references for p53 (phospho Thr55) antibody [ST0439] (GTX01030). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for p53 (phospho Thr55) antibody [ST0439] (GTX01030). Be the first to share your experience with this product.
![Anti-p53 (phospho Thr55) antibody [ST0439] used in Western Blot (WB). GTX01030](/upload/website/prouct_img/small/GTX01030/GTX01030_HM1125_20200214_WB_treatment_Doxorubicin_20031623_836.jpg "Anti-p53 (phospho Thr55) antibody [ST0439] used in Western Blot (WB). GTX01030")
![Anti-p53 (phospho Thr55) antibody [ST0439] used in Western Blot (WB). GTX01030](/upload/website/prouct_img/normal/GTX01030/GTX01030_HM1125_20200214_WB_treatment_Doxorubicin_20031623_836.jpg "Anti-p53 (phospho Thr55) antibody [ST0439] used in Western Blot (WB). GTX01030")