GeneTex
United States (US)

p53DINP1 antibody

Cat No. GTX29775

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

For WB: Use at a dillution of 1 to 2 μg/ml. Human lung tissue lysate can be used as a positive control and a band at 27 kDa can be detected. A lower band at 18 kDa was detected in human spleen, and mouse liver and kidney tissue lysates. Optimal concentrations/dilutions should be determined by the researcher.

Calculated MW

27 kDa. ( Note )

Positive Control

Human lung tissue lysate. Also human spleen, mouse liver and kidney tissue lysates.
PROPERTIES

Form

Liquid

Buffer

PBS, pH 7.2 with 0.02% sodium azide as preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide: amino acids 1-14 of human p53DINP1. This sequence is identical between alpha and beta, and differs by one amino acid from those of mouse.

Purification

Affinity purified with antigen

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Tumor Protein P53 Inducible Nuclear Protein 1,Sip,Tp53Dinp1,Tp53Inp1A,Tp53Inp1B,Teap,P53Dinp1,Tp53Inp1

Background

Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible gene was identified recently and designated p53DINP1 (for p53-dependent damage-inducible nuclear protein 1) and SIP (for stress induced protein) in human and mouse (1,2). A p53DINP1 antisense oligonucleotide inhibits and overexpression of p53DINP1 enhances Ser46 phosphorylation of p53, induction of p53AIP1, and cell death induced by DNA double-strand breaks (1). p53DINP1 may regulate p53-dependent apoptosis through phosphorylation at Ser46 and induction of p53AIP1. The p53DINP1/SIP gene encodes two proteins of 27 and 18 kDa in human and mouse termed p53DINP1-a and p53DINP1-b or SIP27 and SIP18 (1,2). p53DINP1/SIP is expressed in many tissues and induced by a variety of stress agents including UV stress, mutagenic stress, heat shock, and oxidative stress (2).

Database

Research Area