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p53R2 antibody [N1C3]

KO/KD
Anti-p53R2 antibody [N1C3] used in Western Blot (WB). GTX109620
Anti-p53R2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109620
Anti-p53R2 antibody [N1C3] used in Western Blot (WB). GTX109620
Anti-p53R2 antibody [N1C3] used in Western Blot (WB). GTX109620

Cat. No. GTX109620

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all p53R2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

IMR32 , Mouse brain

Predict Reactivity

Rat, Zebrafish, Bovine, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human p53R2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10730756

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ribonucleotide reductase regulatory TP53 inducible subunit M2B , MTDPS8A , MTDPS8B , P53R2

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes the small subunit of a p53-inducible ribonucleotide reductase. This heterotetrameric enzyme catalyzes the conversion of ribonucleoside diphosphates to deoxyribonucleoside diphosphates. The product of this reaction is necessary for DNA synthesis. Mutations in this gene have been associated with autosomal recessive mitochondrial DNA depletion syndrome, autosomal dominant progressive external ophthalmoplegia-5, and mitochondrial neurogastrointestinal encephalopathy. Alternatively spliced transcript variants have been described.

Database

Research Area

DATA IMAGES

Anti-p53R2 antibody [N1C3] used in Western Blot (WB). GTX109620

GTX109620 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX109620 diluted at 1:1000

Anti-p53R2 antibody [N1C3] used in IHC (Paraffin sections) (IHC-P). GTX109620

GTX109620 IHC-P Image

p53R2 antibody [N1C3] detects p53R2 protein at cytoplasm in mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
p53R2 antibody [N1C3] (GTX109620) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-p53R2 antibody [N1C3] used in Western Blot (WB). GTX109620

GTX109620 WB Image

Sample (30 ug of whole cell lysate)
A: IMR32
10% SDS PAGE
GTX109620 diluted at 1:1000

Anti-p53R2 antibody [N1C3] used in Western Blot (WB). GTX109620

GTX109620 WB Image

KO/KD

The data was published in the journal Sci Rep in 2019.PMID: 31519977

REFERENCE

Application Reference

Chen YF et al. Sci Rep 2019; 9 (1) : 13238 Rrm2b deletion causes mitochondrial metabolic defects in renal tubules.
Application : WB
Reactivity : Mouse
Yao-Yu H et al. Oncotarget 2016; (Epub) Systematic discovery of drug action mechanisms by an integrated chemical genomics approach: identification of functional disparities between azacytidine and decitabine.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169