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p54nrb antibody, C-term

Anti-p54nrb antibody, C-term used in Western Blot (WB). GTX89315
Anti-p54nrb antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89315
Anti-p54nrb antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89315
Anti-p54nrb antibody, C-term used in Western Blot (WB). GTX89315

Cat. No. GTX89315

Reference 1

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all p54nrb products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.3μg/ml
IHC-P 3.75μg/ml

Note :

IHC-P
Human Spleen shows nuclear staining in select splenocytes.
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89315-PEP).

Predict Reactivity

Rat, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-NRAAPGAEFAPNK, from the C Terminus of the protein sequence according to NP_031389.3.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

RRID

AB_10721563

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

non-POU domain containing octamer binding , MRXS34 , NMT55 , NRB54 , P54 , P54NRB , PPP1R114

Cellular Localization

Nucleus , Nucleus speckle , Nucleolus

Background

This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16. [provided by RefSeq, Feb 2009]

Database

Research Area

DATA IMAGES

Anti-p54nrb antibody, C-term used in Western Blot (WB). GTX89315

GTX89315 WB Image

WB analysis of mouse brain lysate using GTX89315 p54nrb antibody, C-term.
Dilution : 0.1μg/ml
Loading : 35μg protein in RIPA buffer

Anti-p54nrb antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89315

GTX89315 IHC-P Image

IHC-P analysis of human spleen using GTX89315 p54nrb antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8μg/ml

Anti-p54nrb antibody, C-term used in IHC (Paraffin sections) (IHC-P). GTX89315

GTX89315 IHC-P Image

IHC-P analysis of human testis using GTX89315 p54nrb antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75μg/ml

Anti-p54nrb antibody, C-term used in Western Blot (WB). GTX89315

GTX89315 WB Image

WB analysis of HEK293 overexpressing human p54nrb (mock transfection in first lane) using GTX89315 p54nrb antibody, C-term.

REFERENCE

Application Reference

Li S et al. DNA Repair (Amst) 2017; 51 : 70-78 Cell-type specific role of the RNA-binding protein, NONO, in the DNA double-strand break response in the mouse testes.
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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399