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p54nrb antibody

Anti-p54nrb antibody used in IHC (Paraffin sections) (IHC-P). GTX101419
Anti-p54nrb antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101419
Anti-p54nrb antibody used in IHC (Paraffin sections) (IHC-P). GTX101419
Anti-p54nrb antibody used in Western Blot (WB). GTX101419

Cat. No. GTX101419

Reference 2
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all p54nrb products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Rat, Bovine, Chicken, Xenopus laevis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.72 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human p54nrb. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037534

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

non-POU domain containing octamer binding , MRXS34 , NMT55 , NRB54 , P54 , P54NRB , PPP1R114

Cellular Localization

Nucleus

Background

This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-p54nrb antibody used in IHC (Paraffin sections) (IHC-P). GTX101419

GTX101419 IHC-P Image

p54/nrb antibody detects p54/nrb protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
p54/nrb antibody (GTX101419) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-p54nrb antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101419

GTX101419 ICC/IF Image

p54/nrb antibody detects p54/nrb protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: p54/nrb protein stained by p54/nrb antibody (GTX101419) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Anti-p54nrb antibody used in IHC (Paraffin sections) (IHC-P). GTX101419

GTX101419 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using p54/nrb (GTX101419) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-p54nrb antibody used in Western Blot (WB). GTX101419

GTX101419 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
10% SDS PAGE
GTX101419 diluted at 1:1000

REFERENCE

Application Reference

Chaoui A et al. Hum Mol Genet 2015; 24 (17) : 4933-47 Subnuclear re-localization of SOX10 and p54NRB correlates with a unique neurological phenotype associated with SOX10 missense mutations.
Application : ICC/IF
Reactivity : Human
Chang JF et al. Toxicol Sci 2014; (Epub) nuclear proteomics with xrcc3 knockdown to reveal the development of *-resistant uterine cancer.
Application : WB
Reactivity : Human
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REVIEW

p54nrb antibody Cat. No. GTX101419

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-p54nrb antibody used in Western Blot (WB). GTX101419
Date : Anonymous submitted on 21-Mar-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : T-Rex of HEK293T cells (clone G11)
Amount used : 18 μg
Blocking : 5% skim milk in TBT, 21°C, 1.5Hr
Primary Antibody : 1:1000, 21°C, 1.5Hr
Lane Description : Western blot analysis of the soluble (sup) and insoluble (ppt) fractions of Non-transfected (–) and transfected (+) T-Rex of HEK293T.
Figure Note : Image courtesy of Shintaro Iwashita from Tokushima Bunri University at Kagawa.
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169