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p54nrb antibody

Anti-p54nrb antibody used in Western Blot (WB). GTX101419
Anti-p54nrb antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101419
Anti-p54nrb antibody used in IHC (Paraffin sections) (IHC-P). GTX101419
Anti-p54nrb antibody used in IHC (Paraffin sections) (IHC-P). GTX101419

Cat No. GTX101419

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($319),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji

Predict Reactivity

Rat, Bovine, Chicken, Xenopus laevis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.72 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human p54nrb. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

non-POU domain containing octamer binding , MRXS34 , NMT55 , NRB54 , P54 , P54NRB , PPP1R114

Cellular Localization

Nucleus

Background

This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16. [provided by RefSeq]

Database

DATA IMAGES

Anti-p54nrb antibody used in Western Blot (WB). GTX101419

GTX101419 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
10% SDS PAGE
GTX101419 diluted at 1:1000

Anti-p54nrb antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101419

GTX101419 ICC/IF Image

p54/nrb antibody detects p54/nrb protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: p54/nrb protein stained by p54/nrb antibody (GTX101419) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.

Anti-p54nrb antibody used in IHC (Paraffin sections) (IHC-P). GTX101419

GTX101419 IHC-P Image

p54/nrb antibody detects p54/nrb protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
p54/nrb antibody (GTX101419) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-p54nrb antibody used in IHC (Paraffin sections) (IHC-P). GTX101419

GTX101419 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using p54/nrb (GTX101419) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

REVIEW

p54nrb antibody Cat No. GTX101419

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 21-Mar-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : T-Rex of HEK293T cells (clone G11)
Amount used : 18 μg
Blocking : 5% skim milk in TBT, 21°C, 1.5Hr
Primary Antibody : 1:1000, 21°C, 1.5Hr
Lane Description : Western blot analysis of the soluble (sup) and insoluble (ppt) fractions of Non-transfected (–) and transfected (+) T-Rex of HEK293T.
Figure Note : Image courtesy of Shintaro Iwashita from Tokushima Bunri University at Kagawa.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169