GeneTex

  • Country / Location Selection

United States (US)

p57 Kip2 antibody [57P06]

Anti-p57 Kip2 antibody [57P06] used in IHC (Paraffin sections) (IHC-P). GTX34942

Cat. No. GTX34942

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 57P06

Isotype

 IgG2b

Application

 ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse
Package
100 μg ($529)
See all p57 Kip2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1-2μg/ml
IHC-P 0.25-0.5μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Product Note

Recognizes a protein of 57kDa, identified as p57Kip2. It shows no cross-reaction with p27Kip1.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full-length human p57Kip2 protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BWCR , BWS , CDKN1C , KIP2 , WBS , cyclin dependent kinase inhibitor 1C , p57 , p57Kip2 , p57 Kip2

Cellular Localization

Nucleus

Background

This gene is imprinted, with preferential expression of the maternal allele. The encoded protein is a tight-binding, strong inhibitor of several G1 cyclin/Cdk complexes and a negative regulator of cell proliferation. Mutations in this gene are implicated in sporadic cancers and Beckwith-Wiedemann syndorome, suggesting that this gene is a tumor suppressor candidate. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Oct 2010]

Database

Research Area

DATA IMAGES

Anti-p57 Kip2 antibody [57P06] used in IHC (Paraffin sections) (IHC-P). GTX34942

GTX34942 IHC-P Image

IHC-P analysis of human prostate carcinoma tissue using GTX34942 p57 Kip2 antibody [57P06].

REFERENCE

There are currently no references for p57 Kip2 antibody [57P06] (GTX34942). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for p57 Kip2 antibody [57P06] (GTX34942). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529