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p57 Kip2 antibody

Anti-p57 Kip2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31859
Anti-p57 Kip2 antibody used in Western Blot (WB). GTX31859

Cat. No. GTX31859

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA

Reactivity

 Human
Package
100 μg ($399)
See all p57 Kip2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
ICC/IF 20 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

32 kDa. ( Note )

Positive Control

HEK293 Cell Slide(GTX25524) , HEK293 Cell Lysate(GTX27902)

Product Note

At least three isoforms are known to exist; this antibody will detect all three isoforms. This antibody is predicted to not cross react with other CIP/KIP proteins.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

p57 Kip2 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human p57 Kip2.The immunogen is located within amino acids 40 - 90 of p57 Kip2.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887630

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cyclin dependent kinase inhibitor 1C , BWCR , BWS , KIP2 , WBS , p57 , p57Kip2

Cellular Localization

Nucleus

Background

This gene is imprinted, with preferential expression of the maternal allele. The encoded protein is a tight-binding, strong inhibitor of several G1 cyclin/Cdk complexes and a negative regulator of cell proliferation. Mutations in this gene are implicated in sporadic cancers and Beckwith-Wiedemann syndorome, suggesting that this gene is a tumor suppressor candidate. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Oct 2010]

Database

Research Area

DATA IMAGES

Anti-p57 Kip2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31859

GTX31859 ICC/IF Image

ICC/IF analysis of 293 cells using GTX31859 p57 Kip2 antibody.
Working concentration : 20 μg/ml

Anti-p57 Kip2 antibody used in Western Blot (WB). GTX31859

GTX31859 WB Image

WB analysis of 293 cell lysate using GTX31859 p57 Kip2 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

REFERENCE

Application Reference

Chang MC et al. PLoS One 2016; 11 (11) : e0165438 Effect of Butyrate on Collagen Expression, Cell Viability, Cell Cycle Progression and Related Proteins Expression of MG-63 Osteoblastic Cells.
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399