GeneTex

  • Country / Location Selection

United States (US)

p62Dok antibody [N1C1]

Orthogonal
Anti-p62Dok antibody [N1C1] used in Western Blot (WB). GTX101610
Anti-p62Dok antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX101610
Anti-p62Dok antibody [N1C1] used in Western Blot (WB). GTX101610

Cat. No. GTX101610

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all p62Dok products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

Raji

Predict Reactivity

Mouse, Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human p62Dok. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618614

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

docking protein 1 , P62DOK , pp62

Background

Docking protein 1 is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210(bcr/abl), a chimeric protein whose presence is associated with CML. Docking protein 1 contains a putative pleckstrin homology domain at the amino terminus and ten PXXP SH3 recognition motifs. Docking protein 2 binds p120 (RasGAP) from CML cells. It has been postulated to play a role in mitogenic signaling. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-p62Dok antibody [N1C1] used in Western Blot (WB). GTX101610

GTX101610 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
10% SDS PAGE
GTX101610 diluted at 1:1000

Anti-p62Dok antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX101610

GTX101610 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H661 xenograft, using DOK1(GTX101610) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-p62Dok antibody [N1C1] used in Western Blot (WB). GTX101610

GTX101610 WB Image

Orthogonal

The data was published in the journal Int J Mol Sci in 2018.PMID: 29518977

REFERENCE

Application Reference

Sarin N et al. Int J Mol Sci 2018; 19 (3) key players of * resistance: towards a systems pharmacology approach.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for p62Dok antibody [N1C1] (GTX101610). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169