APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
| Assay dependent |
| Assay dependent |
Not tested in other applications.
Observed MW
75 kDa.
Product Note
This antibody can detect endogenous p63 protein, and it may also recognize over-expressed p73 protein.
Predict Reactivity
Rat, Bovine, Chicken(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.3 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human p63. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2909908
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
tumor protein p63 , AIS , B(p51A) , B(p51B) , EEC3 , KET , LMS , NBP , OFC8 , RHS , SHFM4 , TP53CP , TP53L , TP73L , p40 , p51 , p53CP , p63 , p73H , p73L
Cellular Localization
Nucleus
Background
This gene encodes a member of the p53 family of transcription factors. The functional domains of p53 family proteins include an N-terminal transactivation domain, a central DNA-binding domain and an oligomerization domain. Alternative splicing of this gene and the use of alternative promoters results in multiple transcript variants encoding different isoforms that vary in their functional properties. These isoforms function during skin development and maintenance, adult stem/progenitor cell regulation, heart development and premature aging. Some isoforms have been found to protect the germline by eliminating oocytes or testicular germ cells that have suffered DNA damage. Mutations in this gene are associated with ectodermal dysplasia, and cleft lip/palate syndrome 3 (EEC3); split-hand/foot malformation 4 (SHFM4); ankyloblepharon-ectodermal defects-cleft lip/palate; ADULT syndrome (acro-dermato-ungual-lacrimal-tooth); limb-mammary syndrome; Rap-Hodgkin syndrome (RHS); and orofacial cleft 8. [provided by RefSeq, Aug 2016]
Database
Research Area
DATA IMAGES
![Anti-p63 antibody [HL1027] used in Western Blot (WB). GTX635841](/templates/default/js/lightbox/images/loading.gif "Anti-p63 antibody [HL1027] used in Western Blot (WB). GTX635841")
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GTX635841 WB Image
Non-transfected (–) and transfected (+) A431 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p63 antibody [HL1027] (GTX635841) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX635841 WB Image
Whole zebrafish extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with p63 antibody [HL1027] (GTX635841) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX635841 IHC-P Image
p63 antibody [HL1027] detects p63 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human placenta.p63 stained by p63 antibody [HL1027] (GTX635841) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX635841 IHC-Wm Image
p63 antibody [HL1027] detects p63 protein on whole mount zebrafish by immunohistochemical analysis.Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.Green: p63 stained by p63 antibody [HL1027] (GTX635841) diluted at 1:100.Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC
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GTX635841 IHC-P Image
p63 antibody [HL1027] detects p63 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse testis.p63 stained by p63 antibody [HL1027] (GTX635841) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX635841 ICC/IF Image
p63 antibody [HL1027] detects p63 protein at nucleus by immunofluorescent analysis.Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: p63 stained by p63 antibody [HL1027] (GTX635841) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
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GTX635841 IHC-P Image
p63 antibody [HL1027] detects p63 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tongue.p63 stained by p63 antibody [HL1027] (GTX635841) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX635841 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p63 antibody [HL1027] (GTX635841) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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REFERENCE
There are currently no references for p63 antibody [HL1027] (GTX635841). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for p63 antibody [HL1027] (GTX635841). Be the first to share your experience with this product.
![Anti-p63 antibody [HL1027] used in Western Blot (WB). GTX635841](/upload/website/prouct_img/small/GTX635841/GTX635841_44340_20221104_WB_shRNA_watermark_22110919_252.webp "Anti-p63 antibody [HL1027] used in Western Blot (WB). GTX635841")
![Anti-p63 antibody [HL1027] used in Western Blot (WB). GTX635841](/upload/website/prouct_img/small/GTX635841/GTX635841_44340_20221216_WB_Z_22122018_500.webp "Anti-p63 antibody [HL1027] used in Western Blot (WB). GTX635841")
![Anti-p63 antibody [HL1027] used in IHC (Paraffin sections) (IHC-P). GTX635841](/upload/website/prouct_img/small/GTX635841/GTX635841_45278_20240301_IHC-P_24030600_463.webp "Anti-p63 antibody [HL1027] used in IHC (Paraffin sections) (IHC-P). GTX635841")
![Anti-p63 antibody [HL1027] used in IHC (Whole mount) (IHC-Wm). GTX635841](/upload/website/prouct_img/small/GTX635841/GTX635841_44340_20230203_IHC-Wm_Z_23041719_599.webp "Anti-p63 antibody [HL1027] used in IHC (Whole mount) (IHC-Wm). GTX635841")
![Anti-p63 antibody [HL1027] used in IHC (Paraffin sections) (IHC-P). GTX635841](/upload/website/prouct_img/small/GTX635841/GTX635841_T-44130_20220304_IHC-P_M_w_23061202_810.webp "Anti-p63 antibody [HL1027] used in IHC (Paraffin sections) (IHC-P). GTX635841")
![Anti-p63 antibody [HL1027] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635841](/upload/website/prouct_img/small/GTX635841/GTX635841_T-44130_20210416_ICC_IF_w_23061202_984.webp "Anti-p63 antibody [HL1027] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635841")
![Anti-p63 antibody [HL1027] used in IHC (Paraffin sections) (IHC-P). GTX635841](/upload/website/prouct_img/small/GTX635841/GTX635841_44340_20241004_IHC-P_M_24110700_723.webp "Anti-p63 antibody [HL1027] used in IHC (Paraffin sections) (IHC-P). GTX635841")
![Anti-p63 antibody [HL1027] used in Western Blot (WB). GTX635841](/upload/website/prouct_img/small/GTX635841/GTX635841_45250_20231215_WB_TPM_watermark_24112622_516.webp "Anti-p63 antibody [HL1027] used in Western Blot (WB). GTX635841")
![Anti-p63 antibody [HL1027] used in Western Blot (WB). GTX635841](/upload/website/prouct_img/normal/GTX635841/GTX635841_44340_20221104_WB_shRNA_watermark_22110919_252.webp "Anti-p63 antibody [HL1027] used in Western Blot (WB). GTX635841")
![Anti-p63 antibody [HL1027] used in Western Blot (WB). GTX635841](/upload/website/prouct_img/normal/GTX635841/GTX635841_44340_20221216_WB_Z_22122018_500.webp "Anti-p63 antibody [HL1027] used in Western Blot (WB). GTX635841")
![Anti-p63 antibody [HL1027] used in IHC (Paraffin sections) (IHC-P). GTX635841](/upload/website/prouct_img/normal/GTX635841/GTX635841_45278_20240301_IHC-P_24030600_463.webp "Anti-p63 antibody [HL1027] used in IHC (Paraffin sections) (IHC-P). GTX635841")
![Anti-p63 antibody [HL1027] used in IHC (Whole mount) (IHC-Wm). GTX635841](/upload/website/prouct_img/normal/GTX635841/GTX635841_44340_20230203_IHC-Wm_Z_23041719_599.webp "Anti-p63 antibody [HL1027] used in IHC (Whole mount) (IHC-Wm). GTX635841")
![Anti-p63 antibody [HL1027] used in IHC (Paraffin sections) (IHC-P). GTX635841](/upload/website/prouct_img/normal/GTX635841/GTX635841_T-44130_20220304_IHC-P_M_w_23061202_810.webp "Anti-p63 antibody [HL1027] used in IHC (Paraffin sections) (IHC-P). GTX635841")
![Anti-p63 antibody [HL1027] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635841](/upload/website/prouct_img/normal/GTX635841/GTX635841_T-44130_20210416_ICC_IF_w_23061202_984.webp "Anti-p63 antibody [HL1027] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635841")
![Anti-p63 antibody [HL1027] used in IHC (Paraffin sections) (IHC-P). GTX635841](/upload/website/prouct_img/normal/GTX635841/GTX635841_44340_20241004_IHC-P_M_24110700_723.webp "Anti-p63 antibody [HL1027] used in IHC (Paraffin sections) (IHC-P). GTX635841")
![Anti-p63 antibody [HL1027] used in Western Blot (WB). GTX635841](/upload/website/prouct_img/normal/GTX635841/GTX635841_45250_20231215_WB_TPM_watermark_24112622_516.webp "Anti-p63 antibody [HL1027] used in Western Blot (WB). GTX635841")