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p63 antibody [HL1027]

Anti-p63 antibody [HL1027] used in Western Blot (WB). GTX635841
Anti-p63 antibody [HL1027] used in Western Blot (WB). GTX635841
Anti-p63 antibody [HL1027] used in Western Blot (WB). GTX635841
Anti-p63 antibody [HL1027] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635841
Anti-p63 antibody [HL1027] used in IHC (Paraffin sections) (IHC-P). GTX635841
Anti-p63 antibody [HL1027] used in IHC (Whole mount) (IHC-Wm). GTX635841

Cat. No. GTX635841

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1027

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Wm

Reactivity

Human, Mouse, Zebrafish
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

77 kDa. ( Note )

Predict Reactivity

Rat, Bovine, Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human p63. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2909908

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tumor protein p63 , AIS , B(p51A) , B(p51B) , EEC3 , KET , LMS , NBP , OFC8 , RHS , SHFM4 , TP53CP , TP53L , TP73L , p40 , p51 , p53CP , p63 , p73H , p73L

Cellular Localization

Nucleus

Background

This gene encodes a member of the p53 family of transcription factors. The functional domains of p53 family proteins include an N-terminal transactivation domain, a central DNA-binding domain and an oligomerization domain. Alternative splicing of this gene and the use of alternative promoters results in multiple transcript variants encoding different isoforms that vary in their functional properties. These isoforms function during skin development and maintenance, adult stem/progenitor cell regulation, heart development and premature aging. Some isoforms have been found to protect the germline by eliminating oocytes or testicular germ cells that have suffered DNA damage. Mutations in this gene are associated with ectodermal dysplasia, and cleft lip/palate syndrome 3 (EEC3); split-hand/foot malformation 4 (SHFM4); ankyloblepharon-ectodermal defects-cleft lip/palate; ADULT syndrome (acro-dermato-ungual-lacrimal-tooth); limb-mammary syndrome; Rap-Hodgkin syndrome (RHS); and orofacial cleft 8. [provided by RefSeq, Aug 2016]

Database

Research Area

DATA IMAGES

Anti-p63 antibody [HL1027] used in Western Blot (WB). GTX635841

GTX635841 WB Image

Non-transfected (–) and transfected (+) A431 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p63 antibody [HL1027] (GTX635841) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-p63 antibody [HL1027] used in Western Blot (WB). GTX635841

GTX635841 WB Image

Whole zebrafish extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with p63 antibody [HL1027] (GTX635841) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-p63 antibody [HL1027] used in Western Blot (WB). GTX635841

GTX635841 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p63 antibody [HL1027] (GTX635841) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-p63 antibody [HL1027] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635841

GTX635841 ICC/IF Image

p63 antibody [HL1027] detects p63 protein at nucleus by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: p63 stained by p63 antibody [HL1027] (GTX635841) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Anti-p63 antibody [HL1027] used in IHC (Paraffin sections) (IHC-P). GTX635841

GTX635841 IHC-P Image

p63 antibody [HL1027] detects p63 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
p63 stained by p63 antibody [HL1027] (GTX635841) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-p63 antibody [HL1027] used in IHC (Whole mount) (IHC-Wm). GTX635841

GTX635841 IHC-Wm Image

p63 antibody [HL1027] detects p63 protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: p63 stained by p63 antibody [HL1027] (GTX635841) diluted at 1:100.
Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70ºC

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations