PRODUCT
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:100-1:200 |
|
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 7.8 Tris-EDTA-based Target Retrieval Solution buffer.
This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. The detailed steps by Autostainer please refer to the protocol.
|
Not tested in other applications.
Calculated MW
Positive Control
Tonsil: Virtually all squamous epithelial cells must show a moderate to strong, nuclear staining, while few scattered lymphocytes and endothelial cells must show a at least a weak staining.
Negative Control
Tonsil: The vast majority of lymphocytes should be p63 negative.
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA (Please contact us for PBS only format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment (around aa600-680) of human TP63 protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
tumor protein p63 , AIS , B(p51A) , B(p51B) , EEC3 , KET , LMS , NBP , OFC8 , RHS , SHFM4 , TP53CP , TP53L , TP73L , p40 , p51 , p53CP , p63 , p73H , p73L
Cellular Localization
Nucleus
Background
This gene encodes a member of the p53 family of transcription factors. The functional domains of p53 family proteins include an N-terminal transactivation domain, a central DNA-binding domain and an oligomerization domain. Alternative splicing of this gene and the use of alternative promoters results in multiple transcript variants encoding different isoforms that vary in their functional properties. These isoforms function during skin development and maintenance, adult stem/progenitor cell regulation, heart development and premature aging. Some isoforms have been found to protect the germline by eliminating oocytes or testicular germ cells that have suffered DNA damage. Mutations in this gene are associated with ectodermal dysplasia, and cleft lip/palate syndrome 3 (EEC3); split-hand/foot malformation 4 (SHFM4); ankyloblepharon-ectodermal defects-cleft lip/palate; ADULT syndrome (acro-dermato-ungual-lacrimal-tooth); limb-mammary syndrome; Rap-Hodgkin syndrome (RHS); and orofacial cleft 8. [provided by RefSeq, Aug 2016]
Database
Research Area
DATA IMAGES
![Anti-p63 antibody [MSVA-063R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04458](/templates/default/js/lightbox/images/loading.gif "Anti-p63 antibody [MSVA-063R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04458")
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GTX04458 IHC-P Image
IHC-P analysis of human prostate glands tissue using GTX04458 p63 antibody [MSVA-063R] HistoMAX™.
Strong nuclear p63 positivity in basal cells of prostate glands.
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GTX04458 IHC-P Image
IHC-P analysis of human esophageal squamous cell carcinoma (ESCC) tissue using GTX04458 p63 antibody [MSVA-063R] HistoMAX™.
Esophageal squamous cell carcinoma with intense p63 immunostaining of tumor cells.
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GTX04458 IHC-P Image
IHC-P analysis of human esophagus tissue using GTX04458 p63 antibody [MSVA-063R] HistoMAX™.
A strong p63 staining occurs squamous epithelial cells.
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REFERENCE
There are currently no references for p63 antibody [MSVA-063R] HistoMAX™ (GTX04458). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for p63 antibody [MSVA-063R] HistoMAX™ (GTX04458). Be the first to share your experience with this product.
![Anti-p63 antibody [MSVA-063R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04458](/upload/website/prouct_img/small/GTX04458/GTX04458_20230728_IHC-P_221_23072723_494.webp "Anti-p63 antibody [MSVA-063R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04458")
![Anti-p63 antibody [MSVA-063R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04458](/upload/website/prouct_img/small/GTX04458/GTX04458_20230728_IHC-P_351_23072723_387.webp "Anti-p63 antibody [MSVA-063R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04458")
![Anti-p63 antibody [MSVA-063R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04458](/upload/website/prouct_img/small/GTX04458/GTX04458_20230728_IHC-P_91_23072722_588.webp "Anti-p63 antibody [MSVA-063R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04458")
![Anti-p63 antibody [MSVA-063R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04458](/upload/website/prouct_img/normal/GTX04458/GTX04458_20230728_IHC-P_221_23072723_494.webp "Anti-p63 antibody [MSVA-063R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04458")
![Anti-p63 antibody [MSVA-063R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04458](/upload/website/prouct_img/normal/GTX04458/GTX04458_20230728_IHC-P_351_23072723_387.webp "Anti-p63 antibody [MSVA-063R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04458")
![Anti-p63 antibody [MSVA-063R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04458](/upload/website/prouct_img/normal/GTX04458/GTX04458_20230728_IHC-P_91_23072722_588.webp "Anti-p63 antibody [MSVA-063R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04458")