GeneTex
United States (US)

p70 S6K antibody, C-term

GTX14708_1311_WB_18121410_914.jpg
GTX14708_288_IHC-P_18121410_925.jpg
GTX14708_1312_WB_18121410_510.jpg
GTX14708_1313_WB_18121410_355.jpg

Cat No. GTX14708

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.05-0.1μg/ml
IHC-P 2-4μg/ml

Note :

IHC-P
Human Kidney shows staining in the cytoplasm-rich PCT while less so in the DCT and no signals in the glomerulus.

Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX14708-PEP).

Predict Reactivity

Bovine, Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-MISKRPEHLRMNL, from the C Terminus of the protein sequence according to NP_003152.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ribosomal Protein S6 Kinase B1,Ps6K,S6K,S6K-Beta-1,S6K1,Stk14A,P70 S6Ka,P70(S6K)-Alpha,P70-S6K,P70-Alpha,Rps6Kb1

Background

This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17. [provided by RefSeq, Jan 2013]

Database

Research Area

DATA IMAGES
GTX14708_1311_WB_18121410_914.jpg

GTX14708 WB Image

WB analysis of NIH-3T3 cell lysate using GTX14708 p70 S6K antibody, C-term.
Dilution : 0.1µg/ml
Loading : 35µg protein in RIPA buffer

GTX14708_288_IHC-P_18121410_925.jpg

GTX14708 IHC-P Image

IHC-P analysis of human kidney using GTX14708 p70 S6K antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 2µg/ml

GTX14708_1312_WB_18121410_510.jpg

GTX14708 WB Image

WB analysis of rat brain lysate using GTX14708 p70 S6K antibody, C-term.
Dilution : 1µg/ml
Loading : 35µg protein in RIPA buffer

GTX14708_1313_WB_18121410_355.jpg

GTX14708 WB Image

WB analysis of human placenta lysate using GTX14708 p70 S6K antibody, C-term.
Dilution : 0.05µg/ml
Loading : 35µg protein in RIPA buffer

Package List Price ($)
$ 329