APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
0.05-0.1μg/ml |
2-4μg/ml |
Note :
IHC-P
Human Kidney shows staining in the cytoplasm-rich PCT while less so in the DCT and no signals in the glomerulus.
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Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX14708-PEP).
Predict Reactivity
Bovine, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-MISKRPEHLRMNL, from the C Terminus of the protein sequence according to NP_003152.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ribosomal protein S6 kinase B1 , PS6K , S6K , S6K-beta-1 , S6K1 , STK14A , p70 S6KA , p70(S6K)-alpha , p70-S6K , p70-alpha
Cellular Localization
Mitochondrion outer membrane , Mitochondrion , Cytoplasm
Background
This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17. [provided by RefSeq, Jan 2013]
Database
Research Area
DATA IMAGES
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GTX14708 WB Image
WB analysis of NIH-3T3 cell lysate using GTX14708 p70 S6K antibody, C-term. Dilution : 0.1μg/ml Loading : 35μg protein in RIPA buffer
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GTX14708 IHC-P Image
IHC-P analysis of human kidney using GTX14708 p70 S6K antibody, C-term. Antigen retrieval : citrate buffer pH 6 Dilution : 2μg/ml
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GTX14708 WB Image
WB analysis of rat brain lysate using GTX14708 p70 S6K antibody, C-term. Dilution : 1μg/ml Loading : 35μg protein in RIPA buffer
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GTX14708 WB Image
WB analysis of human placenta lysate using GTX14708 p70 S6K antibody, C-term. Dilution : 0.05μg/ml Loading : 35μg protein in RIPA buffer
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REFERENCE
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REVIEW
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