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p70 S6K antibody

Anti-p70 S6K antibody used in Western Blot (WB). GTX107562
Anti-p70 S6K antibody used in Western Blot (WB). GTX107562
Anti-p70 S6K antibody used in Western Blot (WB). GTX107562
Anti-p70 S6K antibody used in Western Blot (WB). GTX107562
Anti-p70 S6K antibody used in IHC (Paraffin sections) (IHC-P). GTX107562
Anti-p70 S6K antibody used in IHC (Paraffin sections) (IHC-P). GTX107562
Anti-p70 S6K antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107562

Cat No. GTX107562

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

293T , A431 , Jurkat , Raji , HeLa

Predict Reactivity

Rabbit, Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.37 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human p70 S6K. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ribosomal Protein S6 Kinase B1,Ps6K,S6K,S6K-Beta-1,S6K1,Stk14A,P70 S6Ka,P70(S6K)-Alpha,P70-S6K,P70-Alpha,Rps6Kb1

Cellular Localization

Cytoplasm , Cell junction , synapse , synaptosome

Background

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates several residues of the S6 ribosomal protein. The kinase activity of this protein leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding this gene and overexpression of this kinase are seen in some breast cancer cell lines. Alternate translational start sites have been described and alternate transcriptional splice variants have been observed but have not been thoroughly characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-p70 S6K antibody used in Western Blot (WB). GTX107562

GTX107562 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with p70 S6K antibody (GTX107562) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-p70 S6K antibody used in Western Blot (WB). GTX107562

GTX107562 WB Image

Whole cell extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with p70 S6K antibody (GTX107562) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-p70 S6K antibody used in Western Blot (WB). GTX107562

GTX107562 WB Image

S6K antibody detects S6K protein by western blot analysis. Whole cell extracts (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with S6K antibody (GTX107562) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-p70 S6K antibody used in Western Blot (WB). GTX107562

GTX107562 WB Image

Sample (30 µg of whole cell lysate)
A: A431
10% SDS PAGE
GTX107562 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-p70 S6K antibody used in IHC (Paraffin sections) (IHC-P). GTX107562

GTX107562 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using p70 S6K(GTX107562) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-p70 S6K antibody used in IHC (Paraffin sections) (IHC-P). GTX107562

GTX107562 IHC-P Image

p70 S6K antibody detects RPS6KB1 protein at cytoplasm in rat kidney by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
p70 S6K antibody (GTX107562) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-p70 S6K antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107562

GTX107562 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using p70 S6K(GTX107562) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

REFERENCE
REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169