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p70 S6K antibody

Orthogonal
Anti-p70 S6K antibody used in Western Blot (WB). GTX107562
Anti-p70 S6K antibody used in IHC (Paraffin sections) (IHC-P). GTX107562
Anti-p70 S6K antibody used in Western Blot (WB). GTX107562
Anti-p70 S6K antibody used in Western Blot (WB). GTX107562
Anti-p70 S6K antibody used in IHC (Paraffin sections) (IHC-P). GTX107562
Anti-p70 S6K antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107562
Anti-p70 S6K antibody used in Western Blot (WB). GTX107562
Anti-p70 S6K antibody used in Western Blot (WB). GTX107562
Anti-p70 S6K antibody used in Western Blot (WB). GTX107562
Anti-p70 S6K antibody used in Western Blot (WB). GTX107562
Anti-p70 S6K antibody used in IHC (Paraffin sections) (IHC-P). GTX107562
Anti-p70 S6K antibody used in IHC (Paraffin sections) (IHC-P). GTX107562

Cat. No. GTX107562

Reference 9

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all p70 S6K products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Positive Control

HeLa , C8D30 , Raw264.7 , C2C12 , PC-12

Predict Reactivity

Rabbit, Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.7 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human p70 S6K. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951779

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ribosomal protein S6 kinase B1 , PS6K , S6K , S6K-beta-1 , S6K1 , STK14A , p70 S6KA , p70(S6K)-alpha , p70-S6K , p70-alpha

Cellular Localization

Cytoplasm , Cell junction , synapse , synaptosome

Background

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates several residues of the S6 ribosomal protein. The kinase activity of this protein leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding this gene and overexpression of this kinase are seen in some breast cancer cell lines. Alternate translational start sites have been described and alternate transcriptional splice variants have been observed but have not been thoroughly characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-p70 S6K antibody used in Western Blot (WB). GTX107562

GTX107562 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p70 S6K antibody (GTX107562) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-p70 S6K antibody used in IHC (Paraffin sections) (IHC-P). GTX107562

GTX107562 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using p70 S6K(GTX107562) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-p70 S6K antibody used in Western Blot (WB). GTX107562

GTX107562 WB Image

S6K antibody detects S6K protein by western blot analysis. Whole cell extracts (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with S6K antibody (GTX107562) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-p70 S6K antibody used in Western Blot (WB). GTX107562

GTX107562 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
10% SDS PAGE
GTX107562 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-p70 S6K antibody used in IHC (Paraffin sections) (IHC-P). GTX107562

GTX107562 IHC-P Image

p70 S6K antibody detects RPS6KB1 protein at cytoplasm in rat kidney by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
p70 S6K antibody (GTX107562) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-p70 S6K antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107562

GTX107562 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using p70 S6K(GTX107562) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-p70 S6K antibody used in Western Blot (WB). GTX107562

GTX107562 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with p70 S6K antibody (GTX107562) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-p70 S6K antibody used in Western Blot (WB). GTX107562

GTX107562 WB Image

Orthogonal

The data was published in the journal Aging (Albany NY) in 2019. PMID: 31831718

Anti-p70 S6K antibody used in Western Blot (WB). GTX107562

GTX107562 WB Image

Orthogonal

The data was published in the journal Aging (Albany NY) in 2019. PMID: 31831718

Anti-p70 S6K antibody used in Western Blot (WB). GTX107562

GTX107562 WB Image

The data was published in the journal Aging (Albany NY) in 2019. PMID: 31831718

Anti-p70 S6K antibody used in IHC (Paraffin sections) (IHC-P). GTX107562

GTX107562 IHC-P Image

The data was published in the journal Aging (Albany NY) in 2019. PMID: 31831718

Anti-p70 S6K antibody used in IHC (Paraffin sections) (IHC-P). GTX107562

GTX107562 IHC-P Image

The data was published in the journal Aging (Albany NY) in 2019. PMID: 31831718

REFERENCE

Application Reference

Wang H et al. J Cell Mol Med 2020; 24 (2) : 1399-1412 Long non-coding RNA (lncRNA) H19 induces hepatic steatosis through activating MLXIPL and mTORC1 networks in hepatocytes.
Liang S et al. Oncol Lett 2020; 19 (4) : 3197-3204 METTL3 serves an oncogenic role in human ovarian cancer cells partially via the AKT signaling pathway.
Application : WB
Reactivity : Human
Peng Q et al. Aging (Albany NY) 2019; 11 (23) : 11520-11540 Bone morphogenetic protein 4 (BMP4) alleviates hepatic steatosis by increasing hepatic lipid turnover and inhibiting the mTORC1 signaling axis in hepatocytes.
Application : WB
Reactivity : Human
Yaguchi M et al. FEBS Lett 2019; (Epub) The activation mechanism of plant S6 kinase (S6K), a substrate of TOR kinase, is different from that of mammalian S6K.
Liu YH et al. Phytomedicine 2018; 46 : 184-192 Aqueous extracts of Paeonia suffruticosa modulates mitochondrial proteostasis by reactive oxygen species-induced endoplasmic reticulum stress in pancreatic cancer cells.
Application : WB
Reactivity : Human
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Application Reference
Wang H et al. J Cell Mol Med 2020; 24 (2):1399-1412 Long non-coding RNA (lncRNA) H19 induces hepatic steatosis through activating MLXIPL and mTORC1 networks in hepatocytes.
Liang S et al. Oncol Lett 2020; 19 (4):3197-3204 METTL3 serves an oncogenic role in human ovarian cancer cells partially via the AKT signaling pathway.
Application : WB
Reactivity : Human
Peng Q et al. Aging (Albany NY) 2019; 11 (23):11520-11540 Bone morphogenetic protein 4 (BMP4) alleviates hepatic steatosis by increasing hepatic lipid turnover and inhibiting the mTORC1 signaling axis in hepatocytes.
Application : WB
Reactivity : Human
Yaguchi M et al. FEBS Lett 2019; (Epub) The activation mechanism of plant S6 kinase (S6K), a substrate of TOR kinase, is different from that of mammalian S6K.
Liu YH et al. Phytomedicine 2018; 46:184-192 Aqueous extracts of Paeonia suffruticosa modulates mitochondrial proteostasis by reactive oxygen species-induced endoplasmic reticulum stress in pancreatic cancer cells.
Application : WB
Reactivity : Human
Yeh YH et al. J Exp Clin Cancer Res 2018; 37 (1):70 inflammatory * activates hif-1α-mediated epithelial-to-mesenchymal transition via pi3k/akt/mtor pathway.
Application : WB
Reactivity : Human
Tsai JS et al. PLoS One 2016; 11 (1):e0147011 Cadmium Activates Multiple Signaling Pathways That Coordinately Stimulate Akt Activity to Enhance c-Myc mRNA Stability.
Application : WB
Reactivity : Human
Pal SK et al. Mol Cancer Res 2015; 13 (1):130-7 RNA-seq reveals aurora kinase-driven mTOR pathway activation in patients with sarcomatoid metastatic renal cell carcinoma.
Application : IHC-P
Reactivity : Human
Hwang-Verslues WW et al. Oncogene 2011; 30 (21):2463-74 miR-495 is upregulated by E12/E47 in breast cancer stem cells, and promotes oncogenesis and hypoxia resistance via downregulation of E-cadherin and REDD1.
Application : WB
Reactivity : Human
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169