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p70 S6K beta antibody [N1C1]

Anti-p70 S6K beta antibody [N1C1] used in Western Blot (WB). GTX101886
Anti-p70 S6K beta antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101886
Anti-p70 S6K beta antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX101886

Cat. No. GTX101886

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all p70 S6K beta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.72 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human S6K2 / p70 S6K beta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951783

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ribosomal protein S6 kinase B2 , KLS , P70-beta , P70-beta-1 , P70-beta-2 , S6K-beta2 , S6K2 , S6KB , S6KI(2) , S6Kbeta , SRK , STK14B , p70(S6K)-beta , p70S6Kb

Background

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates the S6 ribosomal protein and eucaryotic translation initiation factor 4B (eIF4B). Phosphorylation of S6 leads to an increase in protein synthesis and cell proliferation. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-p70 S6K beta antibody [N1C1] used in Western Blot (WB). GTX101886

GTX101886 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
10% SDS PAGE
GTX101886 diluted at 1:500
Anti-p70 S6K beta antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101886

GTX101886 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using S6K2(GTX101886) antibody at 1:100 dilution.

Anti-p70 S6K beta antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX101886

GTX101886 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using S6K2(GTX101886) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Cho S et al. Mol Cell 2021; 81 (10) : 2064-2075.e8 mTORC1 promotes cell growth via m6A-dependent mRNA degradation.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169