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p70 S6K beta antibody

Anti-p70 S6K beta antibody used in Western Blot (WB). GTX101887
Anti-p70 S6K beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101887
Anti-p70 S6K beta antibody used in Western Blot (WB). GTX101887

Cat No. GTX101887

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , NIH-3T3

Predict Reactivity

Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human S6K2 / p70 S6K beta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ribosomal protein S6 kinase B2 , KLS , P70-beta , P70-beta-1 , P70-beta-2 , S6K-beta2 , S6K2 , S6KB , S6KI(2) , S6Kbeta , SRK , STK14B , p70(S6K)-beta , p70S6Kb

Background

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates the S6 ribosomal protein and eucaryotic translation initiation factor 4B (eIF4B). Phosphorylation of S6 leads to an increase in protein synthesis and cell proliferation. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-p70 S6K beta antibody used in Western Blot (WB). GTX101887

GTX101887 WB Image

Sample (30 ug of whole cell lysate)

A:NIH-3T3
10% SDS PAGE

GTX101887 diluted at 1:1000

Anti-p70 S6K beta antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101887

GTX101887 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using S6K2(GTX101887) antibody at 1:500 dilution.

Anti-p70 S6K beta antibody used in Western Blot (WB). GTX101887

GTX101887 WB Image

Sample(30 μg of whole cell lysate)
A:HeLa S3(GTX14654)
7.5% SDS PAGE
GTX101887 diluted at 1:500

REFERENCE
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REVIEW
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SDS
Glycerol.pdf
Thimerosal.pdf