APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:1000 |
| 1:100-1:200 |
| 1:50-1:100 |
Not tested in other applications.
Calculated MW
Predict Reactivity
Mouse, Rat(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide sequence around phosphorylation site of serine 411 (I-R-S(p)-P-R) derived from human p70 S6K.
Purification
Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ribosomal protein S6 kinase B1 , PS6K , S6K , S6K-beta-1 , S6K1 , STK14A , p70 S6KA , p70(S6K)-alpha , p70-S6K , p70-alpha
Cellular Localization
Mitochondrion outer membrane,Mitochondrion,Cytoplasm
Background
This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17. [provided by RefSeq, Jan 2013]
Database
Research Area
DATA IMAGES
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GTX50294 ICC/IF Image
ICC/IF analysis of methanol-fixed MCF-7 cells using GTX50294 p70 S6K (phospho Ser411) antibody.
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GTX50294 WB Image
WB analysis of extracts from MCF cells untreated or treated with EGF using GTX50294 p70 S6K (phospho Ser411) antibody.
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GTX50294 IHC-P Image
IHC-P analysis of human breast carcinoma tissue using GTX50294 p70 S6K (phospho Ser411) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide
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REFERENCE
There are currently no references for p70 S6K (phospho Ser411) antibody (GTX50294). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for p70 S6K (phospho Ser411) antibody (GTX50294). Be the first to share your experience with this product.
