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p70 S6K (phospho Ser418) antibody

Anti-p70 S6K (phospho Ser418) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32258
Anti-p70 S6K (phospho Ser418) antibody used in Western Blot (WB). GTX32258
Anti-p70 S6K (phospho Ser418) antibody used in IHC (Paraffin sections) (IHC-P). GTX32258

Cat. No. GTX32258

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all p70 S6K products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium Phosphate, 0.87% NaCl, 30% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of p70 S6K. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887645

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ribosomal protein S6 kinase B1 , PS6K , S6K , S6K-beta-1 , S6K1 , STK14A , p70 S6KA , p70(S6K)-alpha , p70-S6K , p70-alpha

Cellular Localization

Mitochondrion outer membrane,Mitochondrion,Cytoplasm

Background

This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17. [provided by RefSeq, Jan 2013]

Database

Research Area

DATA IMAGES

Anti-p70 S6K (phospho Ser418) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32258

GTX32258 ICC/IF Image

ICC/IF analysis of formalin-fixed HeLa cells using GTX32258 p70 S6K (phospho Ser418) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

Anti-p70 S6K (phospho Ser418) antibody used in Western Blot (WB). GTX32258

GTX32258 WB Image

WB analysis of NIH3T3 (A), HeLa (B), KNRK (C) whole cell lysates using GTX32258 p70 S6K (phospho Ser418) antibody.

Anti-p70 S6K (phospho Ser418) antibody used in IHC (Paraffin sections) (IHC-P). GTX32258

GTX32258 IHC-P Image

IHC-P analysis of formalin fixed human breast cancer tissue section using GTX32258 p70 S6K (phospho Ser418) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

REFERENCE

Application Reference

Yim JH et al. Int J Radiat Biol 2017; : 43129 Estimation of low-dose radiation-responsive proteins in the absence of genomic instability in normal human fibroblast cells.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399