APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 - 1:1000 |
| 1:50 - 1:100 |
Not tested in other applications.
Calculated MW
Predict Reactivity
Mouse, Rat(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human p70 S6 Kinase around the phosphorylation site of serine 424 (P-V-Sp-P-V).
Purification
Purified by sequential chromatography on phospho- and non-phospho-peptide affinity columns.
From serum
Conjugation
Unconjugated
RRID
AB_11162120
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
PS6K,RPS6KB1,S6K,S6K1,S6Kbeta1,STK14A,p70 S6KA,p70(S6K)alpha,p70S6K,p70alpha,ribosomal protein S6 kinase B1,p70 S6K,p70 S6 Kinase
Cellular Localization
Cell junction,Synapse,Synaptosome,Mitochondrion outer membrane,Mitochondrion,Nucleus,Cytoplasm
Background
This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17. [provided by RefSeq, Jan 2013]
Database
Research Area
DATA IMAGES
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GTX78978 IHC-P Image
IHC-P analysis of human breast carcinoma tissue using GTX78978 p70 S6K (phospho Ser424) antibody.
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GTX78978 WB Image
WB analysis of 293 cells untreated or treated with serum lysates using GTX78978 p70 S6K (phospho Ser424) antibody.
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REFERENCE
There are currently no references for p70 S6K (phospho Ser424) antibody (GTX78978). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for p70 S6K (phospho Ser424) antibody (GTX78978). Be the first to share your experience with this product.
