GeneTex
United States (US)

p70 S6K (phospho Ser447) antibody

GTX32354_20190131_ICCIF_19013101_126.jpg
GTX32354_20190131_IHC-P_19013101_937.jpg
GTX32354_20190131_WB_19013101_340.jpg

Cat No. GTX32354

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

59 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of p70 S6K. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ribosomal Protein S6 Kinase B1,Ps6K,S6K,S6K-Beta-1,S6K1,Stk14A,P70 S6Ka,P70(S6K)-Alpha,P70-S6K,P70-Alpha,Rps6Kb1

Cellular Localization

Cell junction,Cytoplasm,Mitochondrion,Mitochondrion outer membrane,Nucleus,Synapse,Synaptosome

Background

This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17. [provided by RefSeq, Jan 2013]

Database

Research Area

DATA IMAGES
GTX32354_20190131_ICCIF_19013101_126.jpg

GTX32354 ICC/IF Image

ICC/IF analysis of formalin-fixed A549 cells using GTX32354 p70 S6K (phospho Ser447) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

GTX32354_20190131_IHC-P_19013101_937.jpg

GTX32354 IHC-P Image

IHC-P analysis of formalin fixed human breast cancer tissue section using GTX32354 p70 S6K (phospho Ser447) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

GTX32354_20190131_WB_19013101_340.jpg

GTX32354 WB Image

WB analysis of EGF-treated A549 (A), mouse brain (B), rat brain (C) whole cell lysates using GTX32354 p70 S6K (phospho Ser447) antibody.

Package List Price ($)
$ 319