*Optimal dilutions/concentrations should be determined by the researcher.
|1:500 - 1:1000
|1:100 - 1:500
|1:100 - 1:200
Not tested in other applications.
0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of p70 S6K. The exact sequence is proprietary.
Purified by antigen-affinity chromatography
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Ribosomal Protein S6 Kinase B1,Ps6K,S6K,S6K-Beta-1,S6K1,Stk14A,P70 S6Ka,P70(S6K)-Alpha,P70-S6K,P70-Alpha,Rps6Kb1
Cell junction,Cytoplasm,Mitochondrion,Mitochondrion outer membrane,Nucleus,Synapse,Synaptosome
This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17. [provided by RefSeq, Jan 2013]
GTX32354 ICC/IF Image
ICC/IF analysis of formalin-fixed A549 cells using GTX32354 p70 S6K (phospho Ser447) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes
GTX32354 IHC-P Image
IHC-P analysis of formalin fixed human breast cancer tissue section using GTX32354 p70 S6K (phospho Ser447) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)
GTX32354 WB Image
WB analysis of EGF-treated A549 (A), mouse brain (B), rat brain (C) whole cell lysates using GTX32354 p70 S6K (phospho Ser447) antibody.