APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Rabbit, Bovine, Chicken, Xenopus tropicalis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide surrounding phospho Thr421/Ser424 of human p70 S6K. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2888544
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ribosomal protein S6 kinase B1 , PS6K , S6K , S6K-beta-1 , S6K1 , STK14A , p70 S6KA , p70(S6K)-alpha , p70-S6K , p70-alpha
Cellular Localization
Mitochondrion outer membrane,Mitochondrion,Cytoplasm
Background
This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17. [provided by RefSeq, Jan 2013]
Database
Research Area
DATA IMAGES
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GTX635621 WB Image
Wild-type (WT) and p70 S6K (phospho Thr421/Ser424) knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p70 S6K (phospho Thr421/Ser424) antibody [HL129] (GTX635621) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX635621 IHC-P Image
p70 S6K (phospho Thr421/Ser424) antibody [HL5] detects p70 S6K (phospho Thr421/Ser424) protein at cytoplasm by immunohistochemical analysis in the presence of control peptide (left) or antigen-specific peptide (right).Sample: Paraffin-embedded mouse intestine.p70 S6K (phospho Thr421/Ser424) stained by p70 S6K (phospho Thr421/Ser424) antibody [HL5] (GTX635621) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX635621 WB Image
Untreated (–) and treated (+) Rat2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p70 S6K (phospho Thr421/Ser424) antibody [HL129] (GTX635621) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX635621 WB Image
Untreated (–) and treated (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with p70 S6K (phospho Thr421/Ser424) antibody [HL129] (GTX635621) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX635621 WB Image
Untreated (–) and treated (+) Rat2 whole cell extracts (50 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with p70 S6K (phospho Thr421/Ser424) antibody [HL129] (GTX635621) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX635621 WB Image
Untreated (–) and treated (+) Neuro2A whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with p70 S6K (phospho Thr421/Ser424) antibody [HL5] (GTX635621) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX635621 IHC-P Image
p70 S6K (phospho Thr421/Ser424) antibody [HL5] detects p70 S6K (phospho Thr421/Ser424) protein by immunohistochemical analysis.Sample: Paraffin-embedded rat pancreas.p70 S6K (phospho Thr421/Ser424) stained by p70 S6K (phospho Thr421/Ser424) antibody [HL5] (GTX635621) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX635621 IHC-P Image
p70 S6K (phospho Thr421/Ser424) antibody [HL5] detects p70 S6K (phospho Thr421/Ser424) protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissue.p70 S6K (phospho Thr421/Ser424) stained by p70 S6K (phospho Thr421/Ser424) antibody [HL5] (GTX635621) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
There are currently no references for p70 S6K (phospho Thr421/Ser424) antibody [HL129] (GTX635621). Be the first to share your publications with this product.
REVIEW
p70 S6K (phospho Thr421/Ser424) antibody [HL129]
Cat. No. GTX635621
Rating
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( Average 3 based on 1 users reviews)
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Application
Western Blot(WB)
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( Average 3 based on 1 users reviews)
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Date :
Anonymous submitted on 19-Oct-2021
Application Tested :
WB
Sample Species :
Hu
Sample :
HepG2 cells
Amount used :
25μg
Blocking :
5% non-fat milk, BSA in TBST, 4°C, 1Hr
Primary Antibody :
1:1000, 4°C, 16Hr