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p70 S6K (phospho Thr421/Ser424) antibody [HL129]

Anti-p70 S6K (phospho Thr421/Ser424) antibody [HL129] used in Western Blot (WB). GTX635621
Anti-p70 S6K (phospho Thr421/Ser424) antibody [HL129] used in IHC (Paraffin sections) (IHC-P). GTX635621
Anti-p70 S6K (phospho Thr421/Ser424) antibody [HL129] used in Western Blot (WB). GTX635621
Anti-p70 S6K (phospho Thr421/Ser424) antibody [HL129] used in Western Blot (WB). GTX635621
Anti-p70 S6K (phospho Thr421/Ser424) antibody [HL129] used in IHC (Paraffin sections) (IHC-P). GTX635621
Anti-p70 S6K (phospho Thr421/Ser424) antibody [HL129] used in IHC (Paraffin sections) (IHC-P). GTX635621
Anti-p70 S6K (phospho Thr421/Ser424) antibody [HL129] used in Western Blot (WB). GTX635621
Anti-p70 S6K (phospho Thr421/Ser424) antibody [HL129] used in Western Blot (WB). GTX635621

Cat. No. GTX635621

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL129

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Predict Reactivity

Rabbit, Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Thr421/Ser424 of human p70 S6K. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888544

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ribosomal protein S6 kinase B1 , PS6K , S6K , S6K-beta-1 , S6K1 , STK14A , p70 S6KA , p70(S6K)-alpha , p70-S6K , p70-alpha

Cellular Localization

Mitochondrion outer membrane,Mitochondrion,Cytoplasm

Background

This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17. [provided by RefSeq, Jan 2013]

Database

Research Area

DATA IMAGES

Anti-p70 S6K (phospho Thr421/Ser424) antibody [HL129] used in Western Blot (WB). GTX635621

GTX635621 WB Image

Wild-type (WT) and p70 S6K (phospho Thr421/Ser424) knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p70 S6K (phospho Thr421/Ser424) antibody [HL129] (GTX635621) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-p70 S6K (phospho Thr421/Ser424) antibody [HL129] used in IHC (Paraffin sections) (IHC-P). GTX635621

GTX635621 IHC-P Image

p70 S6K (phospho Thr421/Ser424) antibody [HL5] detects p70 S6K (phospho Thr421/Ser424) protein at cytoplasm by immunohistochemical analysis in the presence of control peptide (left) or antigen-specific peptide (right).
Sample: Paraffin-embedded mouse intestine.
p70 S6K (phospho Thr421/Ser424) stained by p70 S6K (phospho Thr421/Ser424) antibody [HL5] (GTX635621) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-p70 S6K (phospho Thr421/Ser424) antibody [HL129] used in Western Blot (WB). GTX635621

GTX635621 WB Image

Untreated (–) and treated (+) Rat2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p70 S6K (phospho Thr421/Ser424) antibody [HL129] (GTX635621) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-p70 S6K (phospho Thr421/Ser424) antibody [HL129] used in Western Blot (WB). GTX635621

GTX635621 WB Image

Untreated (–) and treated (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with p70 S6K (phospho Thr421/Ser424) antibody [HL129] (GTX635621) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-p70 S6K (phospho Thr421/Ser424) antibody [HL129] used in IHC (Paraffin sections) (IHC-P). GTX635621

GTX635621 IHC-P Image

p70 S6K (phospho Thr421/Ser424) antibody [HL5] detects p70 S6K (phospho Thr421/Ser424) protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat pancreas.
p70 S6K (phospho Thr421/Ser424) stained by p70 S6K (phospho Thr421/Ser424) antibody [HL5] (GTX635621) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-p70 S6K (phospho Thr421/Ser424) antibody [HL129] used in IHC (Paraffin sections) (IHC-P). GTX635621

GTX635621 IHC-P Image

p70 S6K (phospho Thr421/Ser424) antibody [HL5] detects p70 S6K (phospho Thr421/Ser424) protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissue.
p70 S6K (phospho Thr421/Ser424) stained by p70 S6K (phospho Thr421/Ser424) antibody [HL5] (GTX635621) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-p70 S6K (phospho Thr421/Ser424) antibody [HL129] used in Western Blot (WB). GTX635621

GTX635621 WB Image

Untreated (–) and treated (+) Rat2 whole cell extracts (50 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with p70 S6K (phospho Thr421/Ser424) antibody [HL129] (GTX635621) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-p70 S6K (phospho Thr421/Ser424) antibody [HL129] used in Western Blot (WB). GTX635621

GTX635621 WB Image

Untreated (–) and treated (+) Neuro2A whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with p70 S6K (phospho Thr421/Ser424) antibody [HL5] (GTX635621) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

*The competitor is not affiliated with GeneTex and does not endorse this product.

REFERENCE

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REVIEW

p70 S6K (phospho Thr421/Ser424) antibody [HL129] Cat. No. GTX635621

Rating ( Average 3 based on 1 users reviews)
Application
Western Blot(WB) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 19-Oct-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : HepG2 cells
Amount used : 25μg
Blocking : 5% non-fat milk, BSA in TBST, 4°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations