GeneTex
  • Country / Location Selection

United States (US)

p70 S6K (phospho Thr421) antibody

Anti-p70 S6K (phospho Thr421) antibody used in Western Blot (WB). GTX50282
Anti-p70 S6K (phospho Thr421) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50282
Anti-p70 S6K (phospho Thr421) antibody used in IHC (Paraffin sections) (IHC-P). GTX50282

Cat No. GTX50282

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

59 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of threonine 421 (P-R-T(p)-P-V) derived from human p70 S6K.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ribosomal protein S6 kinase B1 , PS6K , S6K , S6K-beta-1 , S6K1 , STK14A , p70 S6KA , p70(S6K)-alpha , p70-S6K , p70-alpha

Cellular Localization

Mitochondrion outer membrane,Mitochondrion,Cytoplasm

Background

This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17. [provided by RefSeq, Jan 2013]

Database

Research Area

DATA IMAGES

Anti-p70 S6K (phospho Thr421) antibody used in Western Blot (WB). GTX50282

GTX50282 WB Image

WB analysis of extracts from Jurkat cells untreated (lane 1) or treated with GM-CSF (lane 2) using GTX50282 p70 S6K (phospho Thr421) antibody.

Anti-p70 S6K (phospho Thr421) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50282

GTX50282 ICC/IF Image

ICC/IF analysis of methanol-fixed MCF cells using GTX50282 p70 S6K (phospho Thr421) antibody.

Anti-p70 S6K (phospho Thr421) antibody used in IHC (Paraffin sections) (IHC-P). GTX50282

GTX50282 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50282 p70 S6K (phospho Thr421) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

There are currently no references for p70 S6K (phospho Thr421) antibody (GTX50282). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for p70 S6K (phospho Thr421) antibody (GTX50282). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf