WB analysis of EGF-treated HepG2 (A), mouse brain (B), rat brain (C) whole cell lysates using GTX32353 p70 S6K (phospho Thr444) antibody.

IHC-P analysis of formalin fixed human breast cancer tissue section using GTX32353 p70 S6K (phospho Thr444) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

ICC/IF analysis of formalin-fixed HepG2 cells using GTX32353 p70 S6K (phospho Thr444) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes