GeneTex

  • Country / Location Selection

United States (US)

p73 antibody [5B429]

Overexpression
Anti-p73 antibody [5B429] used in Western Blot (WB). GTX46123
Anti-p73 antibody [5B429] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX46123
Anti-p73 antibody [5B429] used in IHC (Paraffin sections) (IHC-P). GTX46123
Anti-p73 antibody [5B429] used in Western Blot (WB). GTX46123
Anti-p73 antibody [5B429] used in Flow cytometry (FACS). GTX46123

Cat. No. GTX46123

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5B429

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ChIP assay, MS

Reactivity

 Human, Mouse, Rat
Package
100 μl ($419)
See all p73 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 2 μg/ml
ICC/IF 1:20 - 1:1000
IHC-P 5 - 15 μg/ml
IHC-Fr 5 - 15 μg/ml
FACS 1 μg/ml
IP 1:20 - 1:1000
ChIP assay 1:20 - 1:1000
MS Assay dependent
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Product Note

p73 antibody (clone 5B429) recognizes all of the TAp73 isoforms i.e. isoforms with an intact NH2-terminal transactivation domain (Thottassery et el, 2006, PMID 16505115). Sayan et al 2005 (PMID 15781249) showed that p73 antibody (clone 5B429) reacted with all tested TAp73 isoforms but not deltaNp73 whereas p73 antibody clone 5B1288 showed reactivity against all p73 isoforms including delta Np73. Caspase-cleaved p73 fragments, including 55 kDa and 45 kDa, have been described in literature (Sayan et al, 2008). p73 antibody clone 5B429 has also been referred to as 429 in the literature.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

This antibody was raised against full-length human p73. It reacts with an epitope that is located on the N-terminal region of human p73. It does not cross react with p53.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tumor protein p73 , P73

Cellular Localization

Nucleus,Cytoplasm

Background

This gene encodes tumor protein p73, which is a member of the p53 family of transcription factors involved in cellular responses to stress and development. The family members include p53, p63, and p73 and have high sequence similarity to one another, which allows p63 and p73 to transactivate p53-responsive genes causing cell cycle arrest and apoptosis. The family members can interact with each other in many ways involving direct or indirect protein interactions, resulting in regulation of the same target gene promoters or regulation of each other's promoters. The p73 protein is expressed at very low levels in normal tissues and is differentially expressed in a number of tumors. The p73 gene expresses at least 35 mRNA variants due to the use of alternate promoters, alternate translation initiation sites, and multiple splice variations. Theoretically this can account for 29 different p73 isoforms; however, the biological validity and the full-length nature of most variants have not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-p73 antibody [5B429] used in Western Blot (WB). GTX46123

GTX46123 WB Image

WB analysis of HeLa cell lysate using GTX46123 p73 antibody [5B429].

Anti-p73 antibody [5B429] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX46123

GTX46123 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX46123 p73 antibody [5B429].
Red : primary antibody
Blue : DAPI
Dilution : 3 ug/mL

Anti-p73 antibody [5B429] used in IHC (Paraffin sections) (IHC-P). GTX46123

GTX46123 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX46123 p73 antibody [5B429].
Dilution : 5 μg/ml
Antigen retrieval : 10 mM sodium citrate buffer, pH 6.0

Anti-p73 antibody [5B429] used in Western Blot (WB). GTX46123

GTX46123 WB Image

Overexpression

WB analysis of (A)HeLa overexpressing p73 (B) HeLa mock cell lysate using GTX46123 p73 antibody [5B429].
Dilution : 2 μg/ml

Anti-p73 antibody [5B429] used in Flow cytometry (FACS). GTX46123

GTX46123 FACS Image

FACS (Intracellular staining) analysis of HeLa cells using GTX46123 p73 antibody [5B429].
Blue : Primary antibody
Orange : isotype control
Dilution : 1 μg/mL
Fixation : 4% PFA
Permibilization : 0.1% saponin

REFERENCE

There are currently no references for p73 antibody [5B429] (GTX46123). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for p73 antibody [5B429] (GTX46123). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 419