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p73 (phospho Tyr99) antibody

Anti-p73 (phospho Tyr99) antibody used in Western Blot (WB). GTX32277
Anti-p73 (phospho Tyr99) antibody used in IHC (Paraffin sections) (IHC-P). GTX32277

Cat No. GTX32277

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of p73. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

tumor protein p73 , P73

Cellular Localization

Nucleus,Cytoplasm

Background

This gene encodes a member of the p53 family of transcription factors involved in cellular responses to stress and development. It maps to a region on chromosome 1p36 that is frequently deleted in neuroblastoma and other tumors, and thought to contain multiple tumor suppressor genes. The demonstration that this gene is monoallelically expressed (likely from the maternal allele), supports the notion that it is a candidate gene for neuroblastoma. Many transcript variants resulting from alternative splicing and/or use of alternate promoters have been found for this gene, but the biological validity and the full-length nature of some variants have not been determined. [provided by RefSeq, Feb 2011]

Database

Research Area

DATA IMAGES

Anti-p73 (phospho Tyr99) antibody used in Western Blot (WB). GTX32277

GTX32277 WB Image

WB analysis of Jurkat (A), UV-treated HeLa (B) whole cell lysates using GTX32277 p73 (phospho Tyr99) antibody.

Anti-p73 (phospho Tyr99) antibody used in IHC (Paraffin sections) (IHC-P). GTX32277

GTX32277 IHC-P Image

IHC-P analysis of formalin fixed human lung cancer tissue section using GTX32277 p73 (phospho Tyr99) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

REFERENCE

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REVIEW

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Package List Price ($)
$ 319