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p75 NGF Receptor / CD271 antibody [SPM299]

Anti-p75 NGF Receptor / CD271 antibody [SPM299] used in IHC (Paraffin sections) (IHC-P). GTX34946

Cat. No. GTX34946

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 SPM299

Isotype

 IgG1

Application

 ICC/IF, IHC-P, FACS

Reactivity

 Human, Rabbit, Cat, Monkey, Baboon, Ferret
Package
100 μg ($529)
See all p75 NGF Receptor / CD271 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Product Note

Epitope spans in aa 1-160 of extracellular domain of NGFR/NTR.
We do not recommend use of this product for Mouse,Rat samples.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

NGFR from A875 melanoma cells

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD271 , Gp80LNGFR , NGFR , TNFRSF16 , nerve growth factor receptor , p75(NTR) , p75NTR , p75 NGF Receptor , p75 NTR , p75 NGFR

Cellular Localization

Membrane

Background

Nerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-p75 NGF Receptor / CD271 antibody [SPM299] used in IHC (Paraffin sections) (IHC-P). GTX34946

GTX34946 IHC-P Image

IHC-P analysis of human melanoma tissue using GTX34946 p75 NGF Receptor / CD271 antibody [SPM299].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529