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p75 NGF Receptor / CD271 antibody [SPM299]

Anti-p75 NGF Receptor / CD271 antibody [SPM299] used in IHC (Paraffin sections) (IHC-P). GTX34946

Cat No. GTX34946

Host Mouse
Clonality Monoclonal
Clone Name SPM299
Isotype IgG1
Application ICC/IF, IHC-P
Reactivity Human, Rabbit, Cat, Monkey, Baboon, Ferret
Package
100 μg ($429)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1-2ug/ml
IHC-P 0.5-1ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Specificity/Sensitivity

It recognizes a glycoprotein of 75kDa, identified as low affinity Nerve Growth Factor (NGF) Receptor (p75NGFR) or Neurotrophin Receptor (p75NTR). Its epitope spans in aa 1-160 of extracellular domain of NGFR/NTR. NGF-receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. NGF is important for the development, differentiation, and survival of variety of neuronal and non-neuronal cells. Its action is mediated by binding two distinct receptors, the high affinity p140 and low affinity p75.
PROPERTIES

Form

Liquid

Buffer

10mM PBS with 0.05% BSA, 0.05% azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml

Immunogen

NGFR from A875 melanoma cells

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Nerve Growth Factor Receptor,Cd271,Gp80-Lngfr,Tnfrsf16,P75(Ntr),P75Ntr,Ngfr

Background

Nerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-p75 NGF Receptor / CD271 antibody [SPM299] used in IHC (Paraffin sections) (IHC-P). GTX34946

GTX34946 IHC-P Image

Formalin-fixed, paraffin-embedded human Melanoma stained with NGFR Monoclonal Antibody (SPM299).

REFERENCE
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REVIEW
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Package List Price ($)
$ 429