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p75 NGF Receptor / CD271 antibody

Anti-p75 NGF Receptor / CD271 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102262
Anti-p75 NGF Receptor / CD271 antibody used in IHC (Frozen sections) (IHC-Fr). GTX102262
Anti-p75 NGF Receptor / CD271 antibody used in IHC (Paraffin sections) (IHC-P). GTX102262
Anti-p75 NGF Receptor / CD271 antibody used in Western Blot (WB). GTX102262
Anti-p75 NGF Receptor / CD271 antibody used in Western Blot (WB). GTX102262
Anti-p75 NGF Receptor / CD271 antibody used in Western Blot (WB). GTX102262

Cat No. GTX102262

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

NT2D1 , PC-3 , U87-MG , PC-12 , mouse brain , rat brain

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human p75 NGF Receptor. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

nerve growth factor receptor , CD271 , Gp80-LNGFR , TNFRSF16 , p75(NTR) , p75NTR

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

Nerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-p75 NGF Receptor / CD271 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102262

GTX102262 ICC/IF Image

p75 NGF Receptor antibody detects p75 NGF Receptor protein at cytoplasm by immunofluorescent analysis.
Sample: Culture rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: p75 NGF Receptor protein stained by p75 NGF Receptor antibody (GTX102262) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-p75 NGF Receptor / CD271 antibody used in IHC (Frozen sections) (IHC-Fr). GTX102262

GTX102262 IHC-Fr Image

p75 NGF Receptor antibody detects p75 NGF Receptor protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: p75 NGF Receptor protein stained by p75 NGF Receptor antibody (GTX102262) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-p75 NGF Receptor / CD271 antibody used in IHC (Paraffin sections) (IHC-P). GTX102262

GTX102262 IHC-P Image

p75 NGF Receptor antibody detects p75 NGF Receptor protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
p75 NGF Receptor antibody (GTX102262) diluted at 1:500.

Anti-p75 NGF Receptor / CD271 antibody used in Western Blot (WB). GTX102262

GTX102262 WB Image

Sample (30 ug of whole cell lysate)
A: NT2D1
B: PC-3
C: U87-MG
7.5% SDS PAGE
GTX102262 diluted at 1:2000

Anti-p75 NGF Receptor / CD271 antibody used in Western Blot (WB). GTX102262

GTX102262 WB Image

p75 NGF Receptor antibody detects p75 NGF Receptor protein by western blot analysis. Whole cell extracts (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with p75 NGF Receptor antibody (GTX102262) at a dilution of 1:2000.

Anti-p75 NGF Receptor / CD271 antibody used in Western Blot (WB). GTX102262

GTX102262 WB Image

Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with p75 NGF Receptor / CD271 antibody (GTX102262) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
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REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169