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p75 NGF Receptor / CD271 antibody

Anti-p75 NGF Receptor / CD271 antibody used in Western Blot (WB). GTX10494
Anti-p75 NGF Receptor / CD271 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX10494

Cat No. GTX10494

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-Fr
Reactivity Rat
Package
100 μl ($319)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5,000
ICC/IF 1:500
IHC-Fr 1:10,000

Note :

IHC-Fr
using immersion fixed human peripheral blood lymphocytes

Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

NGF-treated PC12, rat brain
PROPERTIES

Form

Liquid

Buffer

0.01M PBS pH7.4, 1% bovine serum albumin, 15 mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Rat

Immunogen

synthetic peptide corresponding to the C-terminal of NGFR p75 of rat origin (amino acids 407-425 with N-terminally added lysine) conjugated to KLH.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

nerve growth factor receptor , LNGFR , RNNGFRR , Tnfrsf16 , p75 , p75NTR

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

Nerve Growth Factor Receptor, also termed NGFR p75 or p75NTR, is the low-affinity NGFR (LNGFR) which binds NGF and other neurotrophins including BDNF, NT3, NT4/5 with similar, low affinity. NGFR p75 is a 75kD transmembrane glycoprotein (399 a.a.) consisting of an extracellular domain (222 a.a.) which contains four cysteine-rich domains responsible for ligand binding, a transmembrane domain (22 a.a.), and a cytoplasmic domain (155 a.a.). NGFR p75 is mainly expressed in Schwann cells and neurons and in a variety of non-neuronal cells. NGFR p75 is necessary for regulating neuronal growth, migration, differentiation and cell death during development of the central and peripheral nervous system. The signal transduction mechanisms and components leading to NGFR p75 multiple signals are complex and not well understood. In contrast to other members of the neurotrophin receptors family (TrkA, TrkB and TrkC tyrosine kinases), NGFR p75 lacks intrinsic catalytic activity. It has been suggested that NGFR p75 interacts with TrkA to form high affinity binding sites and to modulate TrkA signaling. NGFR p75 belongs to the TNF receptor superfamily which includes TNFR, CD40 and Fas. These receptors all have an intracellular death domain and can couple to parallel signaling pathways leading to apoptotic cell death or activation of the transcription factor NF-kappaB. NGFR p75 plays a central role in the regulation of cell number by apoptosis in the developing CNS. During early development, activation of NGFR p75 by NGF induces apoptotic cell death in some neuronal cells, probably through activation of the sphingomyelinase/ceramide pathway, the ICE-like proteases and the JNK pathway. In rat Schwann cells, NGF binding to NGFR p75 activates NF-kappaB, possibly to modulate Schwann cell migration during nerve regeneration.

Database

Research Area

DATA IMAGES
Anti-p75 NGF Receptor / CD271 antibody used in Western Blot (WB). GTX10494

GTX10494 WB Image

WB analysis of various samples using GTX10494 p75 NGF Receptor / CD271 antibody at 1:5000.
Lane 1: Rat brain
Lane 2: 50 ng/ml NGF-differentiated PC12 cells
Lane 3: Negative control: non-treated PC12 cells

Anti-p75 NGF Receptor / CD271 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX10494

GTX10494 ICC/IF Image

ICC/IF analysis of PC12 cells using GTX10494 p75 NGF Receptor / CD271 antibody at 1:500. Cells were fixed and permeabilized with methanol followed by methanol:acetone.

REFERENCE
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Package List Price ($)
$ 319