GeneTex
United States (US)

p90 RSK1 antibody

GTX105842_40317_IFA_19011506_158.jpg
GTX105842_39946_WB_19011506_446.jpg

Cat No. GTX105842

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.47mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human p90 RSK1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

HU-1 antibody, MAPKAPK1A antibody, RSK antibody, RSK1 antibody, RPS6KA1 antibody, MAPK-activated protein kinase 1a antibody, MAPKAP kinase 1a antibody, p90-RSK 1 antibody, ribosomal S6 kinase 1 antibody, ribosomal protein S6 kinase alpha 1 antibody, dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1) antibody, MAPKAPK-1a antibody, RSK-1 antibody, 90 kDa ribosomal protein S6 kinase 1 antibody, S6K-alpha 1 antibody, MAP kinase-activated protein kinase 1a antibody, ribosomal protein S6 kinase alpha-1 antibody, S6K-alpha-1 antibody, p90S6K antibody, p90RSK1 antibody, ribosomal protein S6 kinase, 90kDa, polypeptide 1 antibody

Background

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX105842_40317_IFA_19011506_158.jpg

GTX105842 ICC/IF Image

p90 RSK1 antibody detects p90 RSK1 protein at Cytoplasm and Nucleus by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: p90 RSK1 protein stained by RSK1 antibody (GTX105842) diluted at 1:500.
Blue: Hoechst 33343 staining.

GTX105842_39946_WB_19011506_446.jpg

GTX105842 WB Image

p90 RSK1 antibody detects p90 RSK1 protein by western blot analysis.
A. 30 µg HepG2 whole cell lysate/extract
7.5% SDS-PAGE
p90 RSK1 antibody (GTX105842) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169