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p90 RSK1 antibody

Anti-p90 RSK1 antibody used in Western Blot (WB). GTX111050
Anti-p90 RSK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX111050
Anti-p90 RSK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111050

Cat. No. GTX111050

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all p90 RSK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human p90 RSK1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951767

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ribosomal protein S6 kinase A1 , HU-1 , MAPKAPK1A , RSK , RSK1 , p90Rsk

Background

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-p90 RSK1 antibody used in Western Blot (WB). GTX111050

GTX111050 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
7.5% SDS PAGE
GTX111050 diluted at 1:10000

Anti-p90 RSK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX111050

GTX111050 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SCM1 xenograft, using p90 RSK1(GTX111050) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-p90 RSK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111050

GTX111050 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using p90 RSK1(GTX111050) antibody at 1:200 dilution.

REFERENCE

Application Reference

Emilie L Cerezo et al. PLoS Genet 2021; 17 (6) : e1009583 RIOK2 phosphorylation by RSK promotes synthesis of the human small ribosomal subunit.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169