GeneTex
United States (US)

p90 RSK1 (phospho Ser221) antibody

Orthogonal
GTX10695_1463_WB_19011519_434.jpg
GTX10695_1007_IHC-P_19011519_212.jpg

Cat No. GTX10695

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Chicken
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
IHC-P 1:10-1:100
Not tested in other applications.

Calculated MW

83 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH 7.3, 50% glycerol, 1mg/ml BSA, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human RSK1 that contains serine 221. The sequence is conserved in mouse and rat.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ribosomal Protein S6 Kinase A1,Hu-1,Mapkapk1A,Rsk,Rsk1,P90Rsk,Rps6Ka1

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX10695_1463_WB_19011519_434.jpg

GTX10695 WB Image

Orthogonal

WB (peptide competition) analysis of untreated A431 (Lane 1) or PMA-stimulated A431 cells (Lane 2~5) using GTX10695 p90 RSK1 (phospho Ser221) antibody prior incubated with the non-phosphopeptide (Lane 3), a generic [pS] peptide (Lane 4), or, the phosphopeptide immunogen (Lane 5) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.

GTX10695_1007_IHC-P_19011519_212.jpg

GTX10695 IHC-P Image

IHC-P analysis of human brain tissue using GTX10695 p90 RSK1 (phospho Ser221) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retireval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

Package List Price ($)
$ 319