IHC-P analysis of human brain tissue using GTX10695 p90 RSK1 (phospho Ser221) antibody.
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retireval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:20

WB (peptide competition) analysis of untreated A431 (Lane 1) or PMA-stimulated A431 cells (Lane 2~5) using GTX10695 p90 RSK1 (phospho Ser221) antibody prior incubated with the non-phosphopeptide (Lane 3), a generic [pS] peptide (Lane 4), or, the phosphopeptide immunogen (Lane 5) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.