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p90 RSK1 (phospho Ser380) antibody

Orthogonal
Anti-p90 RSK1 (phospho Ser380) antibody used in Western Blot (WB). GTX66605
Anti-p90 RSK1 (phospho Ser380) antibody used in Immunoprecipitation (IP). GTX66605
Anti-p90 RSK1 (phospho Ser380) antibody used in Western Blot (WB). GTX66605

Cat. No. GTX66605

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all p90 RSK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IP 1:50 - 1:100
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A phospho specific peptide corresponding to residues surrounding S380 of human P90RSK

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ribosomal protein S6 kinase A1 , HU-1 , MAPKAPK1A , RSK , RSK1 , p90Rsk

Cellular Localization

Nucleus,Cytoplasm

Background

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-p90 RSK1 (phospho Ser380) antibody used in Western Blot (WB). GTX66605

GTX66605 WB Image

Orthogonal

WB analysis of HeLa cells treated by PMA/TPA (200 nM) at 37ºC for 15 minutes after serum-starvation overnight using GTX66605 p90 RSK1 (phospho Ser380) antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-p90 RSK1 (phospho Ser380) antibody used in Immunoprecipitation (IP). GTX66605

GTX66605 IP Image

IP analysis of HeLa cell lysate using GTX66605 p90 RSK1 (phospho Ser380) antibody.
Antibody amount : 3μg / 200μg lysate
Dilution : 1:1000

Anti-p90 RSK1 (phospho Ser380) antibody used in Western Blot (WB). GTX66605

GTX66605 WB Image

Orthogonal

WB analysis of NIH/3T3 cells treated by PMA/TPA (200 nM) at 37ºC for 30 minutes after serum-starvation overnight using GTX66605 p90 RSK1 (phospho Ser380) antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399